ELISA检测单试剂反应性献血者跟踪检测结果分析

Analysis of tracking test results of elisa-single reagent reactive blood donors

目的:了解攀枝花地区酶联免疫吸附试验(ELISA)检测单试剂反应性献血者情况,探讨在攀枝花地区通过献血者追踪检测对单试剂反应性献血者回归的意义,为指导制定相应的献血者归队策略,进一步稳定和扩大献血者队伍提供依据。方法:对2017-01-01-2018-01-31收集的18154例无偿献血者ELISA及核酸检测结果进行统计分析,并对其中单试剂反应性的献血者从检出后第6个月、12个月仍用2次ELISA加1次核酸的方法进行2次跟踪检测。结果:18154例献血者ELISA检测不合格共计264例(1.45%),其中双反应性144例(0.79%),单反应性120例(0.66%)。在对115例单试剂反应性献血者的跟踪检测中有12例(10.4%)ELISA及核酸检测均为非反应性,2例抗-HIV确认为阴性,97例(84.3%)ELISA仍然为单试剂反应性,其余6例(5.2%)转化为双试剂反应性。结论:通过追踪检测提示,在未开展确证实验,不改变检测试剂和检测方法的前提下,跟踪检测对献血者回归、稳定献血者队伍意义不大。但能提示开展核酸检测的情况下,只采用1种ELISA检测试剂进行初筛,可能存在漏检的风险,同时进行2次ELISA检测仍有必要。

Objective:To investigate the situation of single reagent reactive blood donors detected by ELISA in panzhihua area.To explore the significance of tracing blood donors for the return of blood donors in this area.To provide the basis for guiding the formulation of the strategy of returning blood donors,stabilizing and expanding the team of blood donors.Method:The results of ELISA and nucleic acid test in 18154 volunteer blood donors from Jan.1,2017 to Jan.31,2018 were analyzed.The single reagent reactivity blood donors were tracked twice by ELISA plus nucleic acid in the 6 th and 12 th months after detection.Result:A total of 264 cases(1.45%)of 18154 blood donors were not qualified by ELISA,of which 144 cases(0.79%)were bireactivity and 120 cases(0.66%)were monoreactivity.A total of 115 cases were detected.12 cases(10.4%)were non-reactive in ELISA and NAT,97 cases(84.3%)were still single reagent reactivity,and the other 6 cases(5.2%)were converted to double reagent reactivity.Two anti-HIV single reagent reactivity specimens were confirmed negative.Conclusion:Without changing the detection reagents and methods,tracking detection is of little significance to the return of blood donors and the stabilization of blood donors.