广西长寿地区老年人口腔唾液菌群差异性分析

Analysis of difference in oral bacteria flora in saliva among elderly people in longevity zone of Guangxi

目的探讨广西长寿地带巴马长寿老年人和德保老年人口腔唾液细菌种类、特征和菌群差异等,揭示长寿与口腔菌群的关系。方法采集河池市巴马县长寿老年人(BM组)和百色市德保县60岁以上老年人(BS组)口腔唾液,提取细菌总DNA,采用PCR扩增16SrDNA V4区并测序分析,注释唾液标本的主要菌群、菌群多样性,比较分析样本的差异。结果收集BM组与BS组各7例共14组样本,14个样本聚类分析共产生369个OTU;在属水平发现BM组与BS组的优势菌群均为放线菌属、噬二氧化碳细胞菌属、金黄色杆菌属、梭杆菌属、嗜血杆菌属、乳酸杆菌属、纤毛菌属、奈瑟氏菌属、卟啉单胞菌属、普雷沃氏菌属、罗氏菌属、链球菌属、韦荣氏球菌属;主成分分析发现,两组间主成分有差异,但无显著性;多样性分析发现样本间呈现多样性,但差异无统计学意义(P>0.05);物种注释分析和物种热力图分布发现BM组与BS组口腔唾液细菌组成差异不大,在门、纲、目、科、属中乳酸杆菌始终是优势菌群,但在两组中丰度比例不同,BM组口腔中Lactobacillus(乳杆菌属)含量高于BS组,Mycoplasma(支原体)含量低于BS组,且含量差异有统计学意义(P<0.05)。结论BM组与BS组优势菌群均为乳酸杆菌,且BM组高于BS组,因此考虑该地区人群长寿可能与乳酸杆菌有关。

Objective To investigate the bacterial species,characteristics and differences of oral bacteria flora of saliva in the longevous elderly between in Bama county and in Debao county in Guangxi,in order to explore the relationship between longevity and oral salivary bacteria flora in the elderly.Methods The saliva was taken from the longevous elderly in Bama county(BM group)and people aged over 60 years in Debao county(BS group)separately,and the total DNA was extracted.The 16S rDNA-V4 region was amplified by PCR and analyzed by sequencing.The main species and diversity of bacterial colonies were recorded for difference analysis.Results A total of 14 saliva samples were collected from 7 cases in BM group and 7 cases in BS group.A total of 369 OTUs were generated by cluster analysis of 14 samples.At the genus level,the dominant salivary bacteria flora were Ctinomyces,Capnocytophaga,Chryseobacterium,Fusobacterium,Haemophilus,Lactobacillus,Leptotrichia,Neisseria,Porphyromonas,Prevotella,Rothia,Streptococcus,Veillonella in both BM group and BS group.The OTU PCA analysis showed that some evidence for indeterminate differences was found,but statistically significant differences did not exist in the dominant components of oral flora between the two groups(P>0.05).Also,the same tendency toward the diversity(P>0.05)was presented.Similarly,the species annotation analysis and the heat map showed that there were no significant differences(P>0.05)in oral salivary flora composition between the two groups.Lactobacillu was always the prevailing flora in the Phylume,Class,Order,Family and Genus,but the abundance ratio was different between the two groups as following:Lactobacillus abundance in salivary bacteria flora was higher in BM Group than in the BS group,while Mycoplasma abundance was lower in BM Group than in the BS group(P<0.05).Conclusions The dominant salivary bacteria flora is Lactobacillus in both BM and BS group,while,the abundance of Lactobacillus is higher in the BM group than in the BS group,which indicates that the longevity of population in Bama county may be related to Lactobacillus.