益气活血消癥法对良性前列腺增生患者前列腺组织细胞凋亡的干预研究

Intervention of therapy of replenishing qi, activating blood and resolving aggregation on apoptosis of prostate tissue in patients with benign prostatic hyperplasia

目的观察益气活血消癥法对良性前列腺增生(BPH)患者前列腺组织细胞凋亡的影响。方法手术采集BPH患者前列腺组织,进行细胞培养后随机分成空白组,他莫昔芬组(1.00 mg/L),益气活血消癥法高、中、低质量浓度组(6.25、3.13、1.56 mg/L),药物作用24 h后采用TUNEL法检测细胞凋亡指数,流式细胞仪检测细胞周期,RT-PCR法检测bcl-2、bax、caspase-3基因表达水平。结果与空白组比较,益气活血消癥法各组细胞凋亡指数增加,且高、中质量浓度组与他莫昔芬组差异无统计学意义。益气活血消癥法各组G0/G1期细胞较空白组增加,而G2/M期细胞较空白组减少,且均与他莫昔芬组差异无统计学意义,益气活血消癥法高、中质量浓度和他莫昔芬对bcl-2基因的表达有抑制作用,同时增强bax基因的表达。益气活血消癥法各组均能提高caspase-3基因的表达水平。结论益气活血消癥法通过下调bcl-2基因的表达,上调bax基因的表达,提高caspase-3基因的表达水平,将增生的前列腺组织细胞阻滞于G0/G1期,从而促进了细胞凋亡。

Objective To observe the influence of therapy of replenishing qi,activating blood and resolving aggregation on apoptosis of prostate tissue in patients with benign prostatic hyperplasia(BPH).Methods The samples of prostate tissue were collected from BPH patients for cell cultivation,and then divided randomly into blank group,tamoxifen group(1.00 mg/L),high-dose(6.25 mg/L),mid-dose(3.13 mg/L)and low-dose groups(1.56 mg/L)of medicated solution with medicinal used in therapy of replenishing qi,activating blood and resolving aggregation.After medicated for 24 h,the apoptosis index was detected by using TUNEL method,cell cycle was detected by using flow cytometer,and expressions of bcl-2,bax and caspase-3 were detected by using RT-PCR.Results The apoptosis index was promoted in high-dose,mid-dose and low-dose groups,and the difference had no statistical significance among high-dose and mid-dose groups and tamoxifen group.The quantity of G0/G1 phase cells increased and G2/M phase cells decreased in high-dose,mid-dose and low-dose groups compared with blank group,and the difference had no statistical significance among high-dose,mid-dose and low-dose groups and tamoxifen group.The expression of bcl-2 gene was inhibited and meanwhile bax gene expression was promoted in high-dose and mid-dose groups and tamoxifen group.The expression of caspase-3 gene was promoted in high-dose,mid-dose and low-dose groups.Conclusion The therapy of replenishing qi,activating blood and resolving aggregation can improve apoptosis through down-regulating bcl-2 gene expression,up-regulating bax gene expression,promoting caspase-3 gene expression,and blocking proliferated prostate tissue cells at G0/G1 phase.