二补助育汤对胚胎着床障碍模型小鼠子宫内膜自噬基因Beclin-1、LC3B表达的影响

Influence of Erbu Zhuyu Tang on expressions of endometrial autophagy genes-Beclin-1 and LC3B in mice with embryo implantation dysfunction

目的探讨二补助育汤对胚胎着床障碍模型小鼠胚胎着床率及子宫内膜自噬基因Beclin-1、微管相关蛋白轻链3(LC3B)mRNA及蛋白表达的影响。方法将50只妊娠ICR雌性小鼠随机分为空白组、模型组、二补助育汤低剂量组、二补助育汤高剂量组、阿司匹林组,每组10只。用米非司酮造成胚胎着床障碍模型,每组每日以相应药物灌胃,于妊娠第5天下午脱颈处死,观察各组子宫形态,计算各组妊娠率、平均着床位点数,检测子宫内膜Beclin-1、LC3B mRNA及蛋白的表达量。结果空白组小鼠子宫色泽红润,胚胎着床部位膨大,分布均匀;模型组小鼠子宫色白、较细;用药各组子宫较细,色泽淡红。模型组平均着床位点数较空白组减少(P<0.001);与模型组相比,二补助育汤高剂量组与阿司匹林组平均胚胎着床位点数均增多(P<0.05)。模型组小鼠子宫内膜Beclin-1、LC3B mRNA及蛋白表达水平均低于空白组(P<0.05);与模型组相比,二补助育汤低、高剂量组与阿司匹林组Beclin-1、LC3B mRNA及蛋白表达水平均增加(P<0.05)。结论二补助育汤可增加Beclin-1、LC3B mRNA及蛋白的表达,提高子宫内膜细胞自噬水平,从而改善子宫内膜容受性增加平均着床位点数。

Objective To investigate the influence of Erbu Zhuyu Tang(Decoction of Promoting Fertility with Two Tonifying Medicinals)on embryo implantation rate and mRNA and protein expressions of endometrial autophagy genes-Beclin-1 and microtubule-associated protein light chain 3(LC3B)in mice with embryo implantation dysfunction.Methods Female ICR mice(n=50)were randomly divided into blank group,model group,low-dose and high-dose Erbu Zhuyu Tang groups and aspirin group(each n=10).The model of embryo implantation dysfunction was established by using mifepristone.The mice in all groups were orally given corresponding medicinals each day and executed in the afternoon of the 5th d of pregnancy.The morphology of uterus was observed,pregnancy rate and average number of embryo implantation sites were calculated,and mRNA and protein expressions of Beclin-1 and LC3B were detected.Results The color of uterus was ruddy and embryo implantation sites were intumescent and distributed equally in blank group.The color of uterus was pale and slender in model group.The uterus was slender and its color was pink in medicated groups.The number of embryo implantation sites decreased in model group compared with blank group(P<0.001).Compared with model group,the number of embryo implantation sites all increased in high-dose Erbu Zhuyu Tang group and aspirin group(P<0.05).The mRNA and protein expressions of Beclin-1 and LC3B were lower in model group than those in blank group(P<0.05).Compared with model group,the mRNA and protein expressions of Beclin-1 and LC3B increased in low-dose and high-dose Erbu Zhuyu Tang groups and aspirin group(P<0.05).Conclusion Erbu Zhuyu Tang can increase the mRNA and protein expressions of Beclin-1 and LC3B,promote autophagy level of endometrial cells and improve endometrial receptivity and average number of embryo implantation sites.