利咽通窍方对舌下神经损伤后大鼠舌肌收缩功能的作用

Effect of Liyan Tongqiao Fang on tongue muscle contraction in rats after hypoglossal nerve injury

目的采用神经-肌肉电生理方法探讨利咽通窍方对舌下神经损伤后大鼠舌肌收缩功能的作用。方法成年Wistar雄性大鼠60只随机分为3组:假手术组、模型组、利咽通窍方组,每组各20只。模型组和利咽通窍方组建立舌下神经压榨损伤模型,假手术组仅分离舌下神经不压榨。造模后第2天利咽通窍方组开始滴舌利咽通窍方(0.3 mg/d),假手术组和模型组滴舌等量的生理盐水。各组分别于给药第7、14天检测舌左右两侧收缩阈强度、单收缩幅度及强直收缩幅度。结果与同时间点假手术组相比,模型组损伤侧舌肌阈强度升高(P<0.05);与同时间点模型组相比,利咽通窍方组损伤侧舌肌阈强度降低(P<0.05)。与同组的非损伤侧相比,模型组和利咽通窍方组损伤侧舌肌阈强度升高(P<0.05)。与第7天相比,第14天利咽通窍方组和模型组损伤侧舌肌阈强度均降低(P<0.05)。与同时间点假手术组相比,模型组损伤侧舌肌单收缩幅度和强直收缩幅度降低(P<0.01);与同时间点模型组相比,利咽通窍方组舌肌单收缩幅度和强直收缩幅度升高(P<0.05);与同组非损伤侧相比,模型组和利咽通窍方组损伤侧单收缩幅度和强直收缩幅度下降(P<0.05)。第14天利咽通窍方组和模型组损伤侧舌肌单收缩幅度和强直收缩幅度均较第7天升高(P<0.05)。结论利咽通窍方能够降低大鼠舌下神经损伤后的舌肌收缩阈强度,提高舌下神经-舌肌传导系统的兴奋性,提高舌肌的收缩能力,从而改善吞咽困难。

Objective To discuss the therapeutic effect of Liyan Tongqiao Fang(Soothing-throat Freeing-orifice Formula)on tongue muscle contraction in rats after hypoglossal nerve injury by applying nerve-muscle electrophysiology method.Methods Male adult Wistar rats(n=60)were randomly divided into sham-operation group,model group and Liyan Tongqiao Fang group(each n=20).The model of hypoglossal nerve injury was established by clamping in model group and Liyan Tongqiao Fang group,while in sham-operation group hypoglossal nerve was only separated without champing.After modeling for 2 d,Liyan Tongqiao Fang group was given Liyan Tongqiao Fang(0.3 mg/d),and sham-operation group and model group were given the same quantity of saline solution.The threshold strength,single contraction amplitude and tonic contraction amplitude were detected in all groups respectively on the 7th d and the 14th d.Results The threshold intensity of injured tongue muscle increased in model group compared with sham-operation group(P<0.05)at the same time point,and decreased in Liyan Tongqiao Fang group compared with model group(P<0.05)at the same time point.The threshold intensity of injured tongue muscle increased in model group and Liyan Tongqiao Fang group compared with non-injured tongue muscle within the same group(P<0.05).The threshold intensity of injured tongue muscle decreased in Liyan Tongqiao Fang group and model group on the 14th d compared with that on the 7th d(P<0.05).The single contraction amplitude and tonic contraction amplitude of injured tongue muscle decreased in model group compared with sham-operation group(P<0.01)at the same time point,and increased in Liyan Tongqiao Fang group compared with model group(P<0.05)at the same time point.The single contraction amplitude and tonic contraction amplitude of injured tongue muscle decreased in model group and Liyan Tongqiao Fang group compared with non-injured tongue muscle within the same group(P<0.05).The single contraction amplitude and tonic contraction amplitude of injured tongue muscle increased in Liyan Tongqiao Fang group and model group on the 14th d compared with those on the 7th d(P<0.05).Conclusion Liyan Tongqiao Fang can reduce the contraction threshold intensity,promote excitability of hypoglossal nerve-tongue muscle conduction system,improve the contraction ability of tongue muscle,and relieve dysphagia in rats after hypoglossal nerve injury.