树莓根黄酮类化合物的抗氧化活性及其结构分析

Antioxidant activity and structure of flavonoid compounds extracted from raspberry root

目的 分析树莓根黄酮类化合物的抗氧化活性及其结构。方法 采用响应面试验优化超声/微波协同提取树莓根黄酮类化合物的条件;优化后获得的产物经去离子水及20%、40%、60%、80%乙醇进行梯度洗脱纯化,分别测定不同洗脱液对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)及2,2′-联氨-双-3-乙基苯并噻唑啉-6-磺酸(2,2′-azinobis-3-ethylbenzothia zoline-6-sulphonic acid,ABTS)自由基清除能力,并对具有较高抗氧化活性的组分进行结构分析。结果 黄酮类化合物最适提取工艺参数为:微波功率431 W,液料比20. 3∶1,提取温度72. 2℃,提取时间30. 46 min,该条件下获得树莓根提取物得率为(10. 05±0. 2)%。40%、60%及80%乙醇洗脱液中黄酮类化合物纯度可达80%;DPPH及ABTS自由基清除能力IC50分别为等浓度抗坏血酸(Vc)的0. 34和0. 59倍。树莓根黄酮类化合物中主要包括原花青素B1、原花青素C1、儿茶素、(4β,8)-非瑟酮醇-儿茶素、原花青素B2等5种组分。结论 成功分离并鉴定了具有较高抗氧化功能的树莓根黄酮类化合物结构,为树莓根的资源利用提供了理论依据。

Objective To analyze the antioxidant activity and structure of flavonoid compounds extracted from raspberry root.Methods The condition for extraction of flavonoid compounds from raspberry root by ultrasonic/microwave coordinative extraction optimized by response surface test.The product obtained under the optimized condition was purified by gradient elution with deionized water as well as 20%,40%,60%and 80%alcohol.The abilities of various eluents in scavenging 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2’-azinobis-3-ethylbenzothia zoline-6-sulphonic acid(ABTS)radicals were determined,and the compounds with high antioxidant activity were analyzed for structure.Results The optimal power of microwave,ratio of liquid to solid,temperature and time for extraction were 431 W,20.3∶1,72.2℃and 30.46 min respectively.Under the optimized condition,the yield of extract was(10.05±0.2)%,while the purities of flavonoid compounds in the eluents of 40%,60%and 80%alcohol reached 80%.The IC50 of the extract in scavenging DPPH and ABTS radicals were 0.34 and 0.59 times of those of Vc at the same concentration respectively.The flavonoid compounds mainly consisted of five components,i.e.procyanidins B1,procyanidins C1,catechins,fisetinidol-(4β,8)-catechin and procyanidins B2.Conclusion The flavonoid compounds with high antioxidant activities were successfully extracted and identified,which provided a theoretical basis for application of raspberry root.