纳米壳聚糖粒子制备工艺优化及抗菌性研究

Preparation process optimization and antibacterial activity analysis of nano-chitosan particles

以壳聚糖和三聚磷酸钠为原材料,采用离子交联法制备壳聚糖纳米粒子。在单因素实验的基础上,采用响应曲面分析法对纳米壳聚糖制备工艺进行优化,并对其抗菌性进行了研究。最佳制备工艺为:壳聚糖质量浓度0.76 mg/mL,三聚磷酸钠质量浓度0.77 mg/mL,壳聚糖与三聚磷酸钠体积比4.11∶1,壳聚糖溶液pH值5.0。纳米壳聚糖的粒径和Zeta电位的理论预测值分别为122.3 nm和+23.43 mV。验证性实验表明,纳米壳聚糖的粒径为119.2 nm,Zeta电位为+22.8 mV,与理论值偏差分别为2.53%、2.69%,纳米粒子分散均匀。当纳米壳聚糖质量浓度达到0.6 mg/mL时,对大肠杆菌和金黄色葡萄球菌表现出明显抑制作用。该制备工艺为高性能纳米壳聚糖粒子的制备及果蔬保鲜方面的应用提供理论参考。

This study aims to obtain the best preparation technology of nano-chitosan particles and study its antimicrobial activity.Nano-chitosan particles were prepared by the ionic crosslinking method using chitosan and sodium tripolyphosphate as raw materials.Based on the single factor experiment,the preparation process of nano-chitosan particles was optimized by response surface analysis,and its antimicrobial activity was studied.The optimum preparation process is as follows:Chitosan concentration was 0.76 mg/mL,sodium triphosphate concentration was 0.77 mg/mL,the volume ratio of chitosan to sodium triphosphate was 4.11∶1,and the pH value of chitosan solution was 5.0.The theoretical predictions of particle size and Zeta potential of nano-chitosan particles were 122.3 nm and+23.43 mV,respectively.Confirmatory experiments showed that the size of nano-chitosan particles was 119.2 nm and the zeta potential was+22.8 mV,and the deviations from the theoretical values were 2.53% and 2.69% respectively,and nano-chitosan particles were distributed uniformly.Nano-chitosan particles concentration 0.6mg/mL could inhibit E.coli and S.aureus significantly.In conclusion,the preparation process is simple without adding organic reagents;the nano-chitosan particles prepared with this method have a small particle size,uniform distribution and remarkable antimicrobial effect.The experimental results provide a theoretical reference for the preparation and application of nano-chitosan particles.