三种多酚与牛血清蛋白相互作用的初步研究

Study of interactions between three polyphenols and bovine serum albumin

采用紫外光谱法、荧光光谱法、圆二色谱法、同步荧光光谱法等方法分析异荭草素、鞣花酸和安石榴苷与牛血清蛋白(bovine serum albumin,BSA)的相互作用。结果表明,三种多酚类化合物对BSA的荧光猝灭机制主要为静态猝灭;与3种多酚结合后,BSA的构象发生变化,异荭草素与BSA的结合位点比例约为2∶1,安石榴苷、鞣花酸与BSA的结合位点比例约为1∶1;异荭草素、安石榴苷、鞣花酸与BSA相互作用的主要作用力初步确定为疏水作用力,并且此3种多酚与BSA之间的结合主要是吸热和熵驱动的反应。3种多酚均与牛血清蛋白产生不同程度的结合,为蛋白质与多酚复合物的研究提供了理论支撑。

Interactions between three polyphenols and bovine serum protein have been done in this study,which is aimed at providing the basis for the metabolism and transport of polyphenols in vivo.In this study,ultraviolet spectrophotometry,fluorescent spectrophotometry,circular dichroism spectroscopy and synchronous fluorescence spectrophotometry were used to analyze the relationship between isoorientin,ellagic acid,punicalagin and bovine serum albumin(BSA),respectively.The results showed that the fluorescence quenching mechanism of three polyphenols on BSA was mainly static quenching.And combining with three polyphenols,the conformation of BSA was changed.The binding ratio between isoorientin and BSA was approximately 2∶1,while the ratios between punicalagin,ellagic acid and BSA were both 1∶1.Isoorientin,punicalagin,ellagic acid interacted with BSA through hydrophobic force,and the combination of the three kinds of polyphenols and BSA was endothermal and entropy driven reaction.These results could provide theoretical support for the field of protein and polyphenol complexes.