用于五种动物源性成分快速检测的离心式微流控芯片系统研制

Development of centrifugal microfluidic chip system for rapid detection of five animal-derived components

基于离心力驱动原理与重组酶介导扩增技术,研制了一套用于牛、羊、猪、鸭、鸡源性成分快速检测的离心式微流控芯片系统。设计制作了扇形微流控芯片和配套使用的密封盖片、芯片托盘装置,同时搭建了集恒温控制平台、流体控制模块和光电检测模块于一体的便携式微流控速测仪,可实现动物源性成分的核酸恒温扩增及荧光检测。每次可同时检测5个样本,每个样本可同时检测5种指标。考察结果显示,离心式微流控芯片系统可检出牛、羊、猪、鸭、鸡源性成分的最小质量分数分别为1%、1%、0.1%、0.1%、1%,能够在30 min内实现半定量检测,具有快速、自动、高通量的特点,显示了离心式微流控芯片可快速检测肉制品掺伪的潜力。

Based on centrifugal force driving principle and recombinase-aid amplification technology,a centrifugal microfluidic chip system for rapid detection of cattle,sheep,pig,duck and chicken-derived ingredients was developed.The fan-shaped microfluidic chip,the sealing cover and the chip tray were designed and manufactured.A portable microfluidic rapid detection instrument integrating constant temperature control platform,fluid control module and photoelectric detection module was built,which using constant temperature amplification of nucleic acid and fluorescence detection methodology.This instrument can detect 5 samples at the same time,and 5 indicators can be detected simultaneously for each sample.The results showed that the minimum mass fraction detected in cattle,sheep,pig,duck and chicken-derived ingredients by centrifugal microfluidic chip system is 1%,1%,0.1%,0.1%and 1%respectively.The centrifugal microfluidic chip system can realize semi-quantitative detection within 30 min.This system is fast,automatic and high-throughput which showed great potential for rapid detection of adulteration in meat products.