急性髓系白血病患者外周血白细胞长链基因间非编码RNA324(LINC00324)与免疫表型的相关性分析

Correlation analysis between LINC00324 and immunophenotype in peripheral blood leukocytes in patients with acute myeloid leukemia

目的分析急性髓系白血病(AML)患者外周血白细胞长链基因间非编码RNA324(LINC00324)与免疫表型的关系。方法用实时荧光定量PCR和生物信息学数据库分析AML患者外周血白细胞及KG-1、THP-1、U937细胞株中UNC00324的表达水平,并采用Peraon相关分析40例AML患者LINC00324的表达水平与红细胞及血小板计数及LINC00324与免疫表型CD14、CD68、CD64、CDllb、CD4、CD45、CD33、人类白细胞抗原DR(HLA-DR)、CD163、CD2、CD58、CD117、CD43、CD34、CD99、CD8、CD38、CD10、CD13、CD56、CD7、TdT、CD235a、CD138、CD61、髓过氧化物酶(MPO)、CD19的关系。同时选取cBioPortal数据库的数据集(TCGA,NEJM 2013)分析173名AML患者的临床病例数据,分析患者肿瘤样本中LINC00324的表达水平与外周血幼稚细胞百分比及白细胞计数的相关性。结果AML患者外周血白细胞LINC00324表达下调,其表达水平与免疫表型CD33、红细胞及血小板数显著正相关。生物信息学数据库分析显示LINC00324在髓系白血病细胞株中低表达,AML患者中LINC00324表达与CD33、CD117、CDllb、CD14、CD64等多种免疫表型相关,与外周血幼稚细胞百分比及白细胞计数呈负相关。结论UNC00324可能参与调控免疫细胞的分化发育及功能,为AML靶向药物开发或治疗提供新策略。

Objective To investigate the relationship between long-chain intergenic n on-coding RNA324(LINC00324)and immunophenotype in peripheral blood leukocytes of acute myeloid leukemia(AML)patients.Methods Real-time quantitative PCR and bioinformatics databases were utilized to analyze the expression level of LINC00324 in peripheral blood leukocytes and cell lines KG-1,THP-1 and U937 in AML patients.The relationships of the expression level of LINC00324 with the red blood cell and platelet count,the expressi on levels of LINC00324 and immun ophe no types in 40 AML patie nts were analyzed by Person correlation analysis.The immunophenotypes included CD14,CD68,CD64,CDllb,CD4,CD45,CD33,HLA-DR,CD163,CD2,CD58,CD117,CD43,CD34,CD99,CD8,CD38,CD10,CD13,CD56,CD7,TdT,CD235a,CD138,CD61,MPO and CD19.Simultaneously,the cBioPortal database datasets(TCGA,NEJM 2013)were used to analyze the clinical characteristics of 173 AML patients,and to analyze the correlations between the expression level of LINC00324 and the peripheral blood blast percentage and white blood cell count in tumor samples.Results The expression of LINC00324 in peripheral blood leukocytes of AML patients was down-regulated,and its expression level was significantly correlated with immuno phenotype CD33,red blood cell and platelet count.An alysis of bioinformatics database showed that LINC00324 was under-expressed in myeloid leukemia cell lines.The expression of LINC00324 in AML patients was associated with multiple immunophenotypes such as CD33,CD117,CDllb,CD14 and CD64 and was negatively correlated with peripheral blood blast percentage and white blood cell count.Conclusion LINC00324 may be involved in regulating the differentiation,development and function of immune cells,which providing a new strategy for the development of targeted drugs or treatment of AML.