长链非编码RNA OSER1-AS1在非小细胞肺癌细胞中的功能研究

Role of long non-coding RNA OSER1-AS1 in non-small cell lung cancer lines

目的探讨长链非编码RNA(long non-coding RNA, lncRNA)OSER1-AS1在非小细胞肺癌细胞株中的表达及对非小细胞肺癌细胞株生物学行为的影响。方法采用qRT-RCR检测25对肺癌组织和癌旁正常组织中lncRNA OSER1-AS1的表达水平;运用Kaplan-Meier plotter网站分析lncRNA OSER1-AS1的表达与总生存率的关联;设计并构建OSER1-AS1过表达载体,转染构建OSER1-AS1过表达细胞模型;通过CCK-8实验、Transwell实验和流式细胞术检测过表达OSER1-AS1对肺癌细胞增殖、迁移、侵袭和凋亡的影响。结果肺癌组织中lncRNA OSER1-AS1的表达水平显著低于癌旁正常组织(P<0.01)。lncRNA OSER1-AS1低水平表达与较差的预后相关;通过转染成功构建了OSER1-AS1过表达H1299、SPCA1细胞系;过表达OSER1-AS1可以抑制H1299和SPCA1细胞的增殖、迁移和侵袭能力(P<0.01),并促进凋亡(P<0.05)。结论 lncRNA OSER1-AS1可以抑制非小细胞肺癌细胞的增殖、迁移和侵袭能力并促进凋亡,在非小细胞肺癌的发生、发展中可能起到抑癌基因的角色。

Objective To investigate the expression profile of long non-coding RNA(lncRNA) OSER1-AS1 in non-small-cell lung cancer(NSCLC) cell lines and its effect on the biological behaviors in these cells. Methods The expression level of OSER1-AS1 in 25 pairs of lung cancer tissues and adjacent normal tissues was detected by real-time quantitative PCR(qRT-PCR). Survival analysis was conducted by online analysis at Kaplan-Meier plotter website. The overexpression vector of OSER1-AS1 was constructed in plasmid pcDNA3.1 and then transfected into H1299 and SPCA1 cells. The effects of overexpression of OSER1-AS1 on proliferation, migration, invasion and apoptosis of lung cancer cell lines were detected by cell counting kit-8(CCK-8) assay, Transwell assay and flow cytometry, respectively. Results The expression of OSER1-AS1 was significantly lower in lung cancer tissues than paracancerous normal tissue(P<0.01). Kaplan-Meier survival analysis showed that low expression of lncRNA OSER1-AS1 in NSCLC was significantly associated with poor prognosis. After transfection of overexpression vector in the H1299 and SPCA1 cells, the expression of OSER1-AS1 was remarkably enhanced(P<0.01). The overexpression of OSER1-AS1 resulted in the inhibition of proliferation, migration and invasion(P<0.01) and the improvement of apoptosis(P<0.05) of H1299 and SPCA1 cells. Conclusion lncRNA OSER1-AS1 may inhibit the proliferation, migration and invasion(P<0.01), promote the apoptosis(P<0.05) of NSCLC cells, and play a role of tumor-suppressor in oncogenesis and development of the cancer.