乌饭树叶多糖对2型糖尿病大鼠糖脂代谢及抗氧化因子的影响

Effects of Vaccinium Bracteatum Thunb. Leaves Polysaccharide on Glycolipid Metabolism and Antioxidant Factors in Type 2 Diabetes Rats

目的:研究乌饭树叶多糖对2型糖尿病大鼠糖脂代谢及抗氧化因子的影响。方法:通过腹腔注射STZ(20 mg/kg)诱导,高糖高脂饲料喂养建立2型糖尿病大鼠模型。将2型糖尿病模型大鼠随机分为模型对照组、二甲双胍组(320 mg/kg)、乌饭树叶多糖低剂量组(200 mg/kg)、乌饭树叶多糖高剂量组(400 mg/kg)。各组大鼠灌胃给药连续60 d,检测大鼠给药前后的空腹血糖;检测大鼠血清中总胆固醇(TC)、甘油三酯(TG)、游离脂肪酸(FFAs)、丙二醛(MDA)的含量;检测血清中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)的活性;比色法检测大鼠胰腺组织中线粒体呼吸链复合物Ⅰ、Ⅲ活性。结果:乌饭树叶多糖低剂量组、乌饭树叶多糖高剂量组大鼠空腹血糖均低于模型组(P<0.05);乌饭树叶多糖高剂量组大鼠血清TC、TG、FFAs含量均低于模型组(P<0.05);乌饭树叶多糖高剂量组大鼠血清中SOD、CAT活性显著高于模型组,MDA含量显著低于模型组(P<0.05);乌饭树叶多糖高剂量组大鼠胰腺中线粒体呼吸链复合物Ⅰ、Ⅲ的活性明显高于模型组(P<0.05)。结论:乌饭树叶多糖可降低2型糖尿病大鼠的空腹血糖,调节改善糖脂代谢,提高抗氧化能力。

Objective: To investigate effects of Vaccinium bracteatum Thunb. leaves polysaccharide on glycolipid metabolism and antioxidant factors in Type 2 diabetes rats. Methods: Rats were injected with streptozotocin(STZ), 20 mg/kg, and fed with high glucose and high fat diet to establish diabetes. And the diabetic rats were assigned into model control group, metformin control group(320 mg/kg), and Vaccinium bracteatum Thunb.leaves polysaccharide low(200 mg/kg) and high dose groups(400 mg/kg) of groups. Rats were orally administered for 60 days;the fasting blood glucose(FBG) was monitored. Rats were sacrificed after last administration, then the serum levels of total cholesterol(TC), triglycerides(TG), free fatty acids(FFAs), malondialdehyde(MDA), superoxide dismutase(SOD) and catalase(CAT) were determined. Mitochondrial respiratory chain complex Ⅰ andⅢ activity were detected by colorimetric method. Results: Vaccinium bracteatum Thunb. leaves polysaccharide low and high dose groups showed lower FBG than model control group(P <0.05). The Vaccinium bracteatum Thunb. leaves polysaccharide high dose group showed lower TC, TG, MDA and FFAs levels than model control group(P<0.05). The Vaccinium bracteatum Thunb. leaves polysaccharide high dose group showed higher activities of SOD and CAT than model control group, while lower MDA than model control(P<0.05). The Vaccinium bracteatum Thunb. leaves polysaccharide high dose group showed higher mitochondrial respiratory chain complexⅠ and Ⅲ were than model control(P<0.05). Conclusion: Vaccinium bracteatum Thunb. leaves polysaccharide can reduce FBG of diabetic rats, regulate and improve the glycolipid metabolism, and improve the antioxidant capacity.