GPR30在胚胎停育早孕绒毛组织中的表达及其意义

Expression and Significance of GPR30 in Chorionic Villi of Early Damaged Embryos

【目的】探讨G蛋白耦联受体3O(GPR3O)在早孕胚胎停育绒毛组织中的表达及其与雌激素受体(ER)、孕激素受体(PR)的相关性。【方法】选取2017年3月至2018年3月在长沙市第一医院行人工流产的胚胎停育患者(观察组)及正常早孕要求终止妊娠着(对照组)餉绒.毛组织各50例,采用免疫组织法检测绒毛组织中GPR30、ER、PR的表达水平(光密度值),并分析三者的相关性。【结果】对照组绒毛组织中GPR30、ER、PR表达水平分别为0.37±0.07、0.34±0.07、0.30±0.05,均显著高于观察组的0.30±0.06,0.28±0.06、0.27±0.04,其差异有统计学意义(P<0.05)。在胚胎停育组孕妇绒毛组织中ER与PR表达呈正相关性(r=0.486,P=0.000);GPR30与ER、PR表达均无明显相关性(r=-0.120,P=0.252;r=-0.027,P=0.794)。【结论】GPR30、ER、PR在早孕胚胎停育绒毛组织中具有一定量表达,但较正帯早孕人工流产绒毛组织的表达显著降低。胚胎停育组孕妇绒毛组织中GPR30与ER、PR表达无明显相关性;ER与PR呈正相关性,说明两者在维持妊娠过程中互相彩响。

【Objective】To investigate the expression of G protein-couple receptor(GPR30)in chorionic villi of early pregnancy embryo damage and its correlation with estrogen receptor(ER)and progesterone receptor(PR).【Methods】A total of 100 cases of chorionic villous tissues from March 2017 to March 2018 were selected from the First Hospital of Changsha.There were 50 cases with embryo damage for abortion(the observation group)and 50 cases of normal early pregnancy requiring pregnancy termination(the control group).The expression level of GPR30,ER and PR in villus tissue was detected by immunohistochemical staining and the average optical density value of staining was measured.【Results】The average optical density values of GPR30,ER and PR staining in the villus tissue of normal early pregnancy group were 0.37±0.07,0.34±0.07 and 0.30±0.05,respectively.The average optical density values of GPR30,ER and PR staining in the embryo damage group were 0.30±0.06,O.28±O.O6 and 0.30±0.04,respectively.The expression levels of GPR30,ER and PR in villus tissue of the normal early pregnancy group were higher than those in the embryo damage group;and the differences were statistically significant(P<0.05).There was a positive correlation between ER and PR expression in the villous tissue of pregnant women with embryo damage for abortion(r=0.486,P=0.000);however,there was no significant correlation of GPR30 with ER and PR expression(r=—0.120,P=0.252;r=—0.027,P=0.794).【Conclusion】The levels of GPR30,ER and PR expression in early pregnancy chorionic villus tissue of abortion caused by embryo damage are significantly lower than those in normal chorionic villi of early pregnancy.There is no significant correlation of GPR30 with ER and PR in the villous tissue of the two groups of pregnant women.The expression of ER shows a positive correlation with PR expression in early pregnancy villus tissue,indicating that the two hormone receptors interact with each other in the process of maintaining pregnancy.