摘要
目的采用离子色谱法测定利多卡因氯己定气雾剂中苯扎溴铵的含量。方法采用AS18色谱柱(250 mm×4 mm),检测器为电导检测器,流动相为15 mmol·L^-1氢氧化钾溶液,流速1 mL·min^-1,柱温35℃。结果1.09~130.80μg·mL^-1苯扎溴铵与色谱峰面积呈良好的线性关系,回归方程为:Y=0.0284X-0.012(r=0.9997),回收率为99.7%,RSD=1.20%。结论所用方法简便、快速,可准确地测定利多卡因氯己定气雾剂中苯扎溴铵的含量,为全面控制利多卡因氯己定气雾剂的质量提供了可靠的检测方法。
OBJECTIVE To determine the benzalkonium bromide in Lidocaine and chlorhexine acetate aerosol by Ion chromatography.METHODS The assay was performed on AS18 column(250 mm×4 mm)by the conductivity detector.The mobile phase was 15 mmol·L^-1 potassium hydrexide solution,the flow rate was 1 mL·min^-1,and the column temperature was 35℃.RESULTS There was a good linearity between peak area and concentration(Y=0.0284X-0.012,r=0.9997)in the range of 1.09-130.8μg·mL^-1 with the average recovery of 99.7% and the RSD of 1.20%.CONCLUSION The simple and rapid method can be used to determine the content of benzalkonium bromide in Lidocaine and chlorhexine acetate aerosol accurately.
作者
钱忠义
蔡鹏
吴珺
QIAN Zhongyi;CAI Peng;WU Jun(Taizhou Institute for Food and Drug Control,Taizhou,Jiangsu,225300 P.R.China)
出处
《华西药学杂志》
CAS
CSCD
2020年第1期88-90,共3页
West China Journal of Pharmaceutical Sciences
关键词
利多卡因氯己定气雾剂
苯扎溴铵
苯扎氯铵
无机阴离子
离子色谱
电导检测器
含量测定
质量控制
Lidocaine and chlorhexine acetate aerosol
Benzalkonium bromide
Benzalkonium chloride
Inorganic anion
Ion chromatography
Electrical conductivity detector
Assay
Quality control