miR-200 a联合糖类抗原125、白介素-17检测在上皮性卵巢癌早期诊断中的价值

The value of detection of microRNA-200 a combined with CA 125 and IL-17 in early diagnosis of epithelial ovarian cancer

目的探讨血浆miR-200 a、糖类抗原125(carbohydrate antigen 125,CA125)、白介素-17(interleukin-17,IL-17)联合检测在早期上皮性卵巢癌中的诊断价值。方法选取2015年8月至2018年8月唐山市妇幼保健院收治的卵巢肿瘤患者128例为研究对象,其中上皮性卵巢癌59例,卵巢良性肿瘤69例,选取同期体检健康者74例为对照组,比较3组受试者血浆miR-200 a、CA125、IL-17水平差异,并分析3者与上皮性卵巢癌临床病理组织类型及临床分期关系,应用受试者工作曲线(receiver operating characteristic curve,ROC)分析血清miR-200 a、CA125、IL-17水平以及3者联合对上皮性卵巢癌的诊断价值。结果上皮性卵巢癌组血浆miR-200 a、CA125、IL-17水平高于卵巢良性肿瘤组与对照组,差异均有统计学意义(P<0.05);卵巢良性肿瘤组与对照组比较差异无统计学意义(P>0.05)。与中、高分化、TNM分期Ⅰ期相比,低分化、TNM分期Ⅱ期患者血浆miR-200 a、CA 125、IL-17表达显著升高(P<0.05);ROC曲线显示,血浆miR-200 a、CA125、IL-17及3者联合检测预测早期上皮性卵巢癌的曲线下面积分别为0.793(敏感度为83.1%,特异性为82.6%)、0.820(敏感度为89.7%,特异性为71.00%)、0.889(敏感度为91.5%,特异性为79.7%)、0.943(敏感度为94.9%,特异性为84.1%)。结论上皮性卵巢癌患者血浆miR-200 a、CA125、IL-17水平升高,3者联合检测可能作为上皮性卵巢癌的早期诊断指标。

Objective To investigate the diagnostic value of combined detection of plasma miR-200 a,carbohydrate antigen125(CA 125)and interleukin 17(IL-17)in early epithelial ovarian cancer.Methods 128 patients with ovarian tumors admitted in Maternal and Child Health Hospital of Tangshan from August 2015 to August 2018 were selected,including 59 cases of epithelial ovarian cancer and 69 cases of benign ovarian tumors.Another 74 healthy persons were selected as the control group during the same period.The levels of plasma miR-200 a,CA 125 and IL-17 were compared among the three groups,and their relationship with the clinical pathological types and clinical stages of epithelial ovarian cancer were analyzed,the diagnostic values of Serum levels of miR-200 a,CA125,IL-17 and their combination for epithelial ovarian cancer were analyzed by receiver operating curve(ROC).Results The levels of plasma miR-200 a,CA 125,and IL-17 in the epithelial ovarian cancer group were higher than those in the benign ovarian tumor group and the control group,and the differences were statistically significant(P<0.05).There was no significant difference between the benign ovarian tumor group and the control group(P>0.05).Compared with moderately,highly differentiated,and TNM stageⅠ,plasma miR-200 a,CA 125,and IL-17 expressions in patients with low differentiated and TNM stageⅡwere significantly increased(P<0.05);The ROC curve showed that the areas under the curve for the prediction of early epithelial ovarian cancer by combined detection of plasma miR-200 a,CA 125,IL-17 and 3 were 0.793(sensitivity 83.1%,specificity 82.6%),0.820(Sensitivity is 89.7%,specificity is 71.00%),0.889(sensitivity is 91.5%,specificity is 79.7%),0.943(sensitivity is 94.9%,specificity is 84.1%).Conclusion Patients with epithelial ovarian cancer have elevated levels of miR-200 a,CA 125,and IL-17.The combined detection of these three may be used as an early diagnostic indicator of epithelial ovarian cancer.