响应面法优化白灵菇菌渣漆酶降解除草剂异丙隆

Optimization of the Degradation of Herbicide Isoproturon by Pleurotus tuoliensis Cultivating Residue Laccase Using Response Surface Methodology

以白灵菇菌渣漆酶对除草剂异丙隆的降解为研究对象,在单因素试验的基础上,采用Box-Behnken试验设计及响应面分析法对白灵菇菌渣漆酶降解异丙隆的条件进行优化,以期获得最佳反应条件。结果表明:反应温度、pH及反应体系体积是影响异丙隆降解的3个主要因素。最佳降解条件为温度37℃,pH 6.8,反应体系体积5.0 mL,在该条件下进行3次平行试验验证,白灵菇菌渣漆酶对异丙隆降解率为78.09%,与预测值基本相符,证明该模型可行。

On the basis of single factor,the optimization of the degradation of herbicide isoproturon by Pleurotus tuoliensis cultivating residue laccase using response surface methodology was investigated,in order to obtain the best reaction conditions.The results showed that temperature,pH and volume of reaction system were the three main factors affecting the degradation of isoproturon.The optimal conditions for isoproturon degradation was determined as follows,temperature 37℃,pH 6.8 and volume of the reaction system 5.0 mL.In a verification experiment,the average degradation rate of isoproturon was 78.09%,it was basically consistent with the predicted value,which proved that the model was feasible.