可溶性程序性死亡因子-1在慢性丙型肝炎患者血清中的变化及意义

Changes and significance of serum soluble programmed death-1 in patients with chronic hepatitis C

目的研究血清中可溶性程序性死亡因子-1(sPD-1)在慢性丙型肝炎患者中的变化及意义,探讨其在慢性丙型肝炎疾病进展中的作用。方法采用ELISA技术检测样本血清中sPD-1的水平,对比在健康对照组和慢性丙型肝炎组之间的差异,分析慢性丙型肝炎患者血清中sPD-1与外周血HCV RNA、谷丙转氨酶、谷草转氨酶及肝纤维化程度(APRI指数)的相关性。结果慢性丙型肝炎患者血清中sPD-1水平高于健康对照组血清中sPD-1水平,差异有统计学意义(P<0.05),且慢性丙型肝炎组sPD-1水平与HCV RNA、谷丙转氨酶及谷草转氨酶水平均呈正相关(P<0.05)。此外,APRI指数≥1.2的慢性丙型肝炎患者(明显肝纤维化组)的血清sPD-1水平较无或者轻度肝纤维化的慢性丙型肝炎患者更高(P<0.05)。结论sPD-1在慢性丙型肝炎的发展中可能发挥了一定的作用。检测慢性丙型肝炎患者体内血清sPD-1水平有助于判断患者的病情进展,对患者病情诊断和临床治疗决策有一定指导意义。

Objective To analyze the changes and significance of serum soluble programmed death-1(sPD-1)in patients with chronic hepatitis C(CHC)and study its role in the progression of CHC.Methods Serum levels of sPD-1 in CHC patients and healthy controls(HC)were measured using ELISA and compared.Correlations of serum sPD-1 with peripheral hepatitis C virus(HCV)RNA,alanine aminotransferase(ALT),aspartate aminotransferase(AST)and liver fibrosis(indicated by APRI)were analyzed.Results The serum sPD-1 level in the CHC group was significantly higher than that in the HC group(P<0.05),and positively correlated with peripheral HCV RNA,ALT and AST(P<0.05).In addition,the serum sPD-1 levels in patients with APRI greater than 1.2(indicating severe liver fibrosis)were higher than those in patients without or with mild liver fibrosis(P<0.05).Conclusions sPD-1 might be involve in the progression of CHC.Measuring serum sPD-1 in CHC patients would assist the prediction of disease progression and help to make the correct diagnosis and appropriate clinical therapy decision.