多倍化兰州百合和细叶百合组培苗再生和耐非生物胁迫能力

Effects of Regeneration and Abiotic Stress Tolerance in Polyploid Lilium davidii var. unicolor and Lilium pumilum in Vitro

以兰州百合(Lilium davidii var.unicolor)和细叶百合(Lilium pumilum DC.Fisch.)二倍体及其同源多倍体组培苗为试材,比较离体培养不同倍性百合植株再生能力以及抗旱、抗盐碱能力的差异。结果表明:相比于二倍体,四倍体鳞片形成不定芽和体细胞胚的时间显著缩短,且再生苗形态硕大,倍性稳定。非整倍体鳞片再生能力显著降低,产生不具有胚性的愈伤组织。混倍体鳞片再生植株形态差异大,性状不稳定,诱导的体细胞胚逐渐褐化死亡。分别利用PEG-6000模拟干旱胁迫和NaHCO3模拟盐碱胁迫,四倍体植株保持较好的适应性,二倍体植株则更为敏感。两种百合四倍体植株通过延伸根系长度并维持叶片良好的水分平衡来保持其逆境下的稳定性。

In order to clarify the growth characteristics of the new polyploid germplasm in Lilium,variation of plant regeneration ability,drought resistance and saline-alkali resistant ability were compared between the diploid seedlings and its homologous polyploid seedlings in vitro in Lilium davidii var.unicolor and Lilium pumilum DC.Fisch.The results showed that the duration for the formation of adventitious buds and somatic embryos in the tetraploid plants scales were significantly shortened and the regeneration seedlings were larger with the stable ploidy compared with the diploid plant scales.The regeneration ability of the aneuploid scales was reduced significantly,and the callus without embryo ability was produced.The regenerated plants produced by the mixoploid scales have different morphology and unstable traits,and the induced somatic embryos gradually brown during culture.PEG-6000 and NaHCO3 were used to simulate drought stress and saline-alkali stress,respectively.Under low concentration stress,the tetraploid seedlings kept good adaptability,while the diploid seedlings were more sensitive.The tetraploid plants of both lily species maintain their stability under stress by extending roots length and keeping a good water balance in leaves.