精液细胞学检查对梗阻性无精子症的诊断作用

Role of semen cytology in the diagnosis of obstructive azoospermia

目的:探讨精液细胞学检查对梗阻性无精子症的诊断作用。方法:将40例无精子症患者(精液镜检可见大量圆形细胞)根据睾丸活检结果分为两组,30例梗阻性无精子症组和10例非梗阻性无精子症组。对照组为40例正常生育男性。采用H-E染色方法对患者精液脱落细胞进行染色,并进行细胞分类。组间两两比较细胞分类结果的差异。结果:梗阻性无精子症组精液中性粒细胞百分率为83.5%,非梗阻性无精子症组精液中性粒细胞为5.40%,对照组精液中性粒细胞为4.83%,梗阻性无精子症组精液中性粒细胞百分率明显高于非梗阻性无精子症组和对照组,两组比较差异具有统计学意义(P<0.001)。结论:精液细胞学检查对梗阻性无精子症(镜下可见大量圆形细胞)的诊断有着研究的价值。

Objective:To investigate the diagnostic effect of semen cytology on obstructive azoospermia.Methods:40 patients with azoospermia(a large number of round cells were detected by semen microscopy)were divided into two groups according to testicular biopsy results,30 patients were with obstructive azoospermia and 10 patients with non-obstructive azoospermia.And another 40 normal fertile men were included in the control group.The patient's semen exfoliated cells were stained by H・E staining and cell sorting was performed.Differences in cell classification results were compared between groups.Results:The percentage of semen neutrophils in the obstructive azoospermia group was 83.5%,5.40%for nonobstructive azoospermia group,and 4.83%for normal men in the control group.The percentage of semen neutrophils in the obstructive azoospermia group was significantly higher than that in the non・obstructive azoosperm group and the control group,which were of statistical differences(P<0.001).Conclusion:Semen cytology has a research value in the diagnosis of obstructive azoospermia(a large number of round cells can be detected under the microscope).