HPLC法测定藤黄药材中藤黄酸的含量

Determination of gambogic acid in gamboge by HPLC

目的:建立HPLC法测定不同产地藤黄药材中藤黄酸的含量。方法:HPLC分析采用ZORBAX SB-C8(150mm×4.6mm,5μm)色谱柱,以乙腈-0.3%的甲酸(65:35)为流动相;流速1mL/min,检测波长为360nm。结果:藤黄酸分离良好,质量浓度在18.56~185.56μg/mL范围内与峰面积线性关系良好,回归方程为Y=23259X+945(R^2=0.9999,n=6),重现性良好,平均加样回收率为100.00%,RSD为1.03%(11=9),6批不同产地藤黄药材中藤黄酸占药材量的平均含量为21.39%。结论:本研究建立的HPLC方法稳定可靠、科学、简便、专属性较强,可用于藤黄药材中藤黄酸的定量,为藤黄的质量评价和质量控制奠定了基础。

Objective:To establish an HPLC method for the determination of gambogic acid in gamboge from different habitats.Method:Column was ZORBAX SB-C8(150mm×4.6mm,5μm),and the mobile phase was acetonitrile-0.3%formic acid(65:35),with flow rate lmL/min,detection wavelength 360nm.Results:gambogic acid was isolated Good,the mass concentration is in the range of 18.56〜185.56μg/mL,and the linear relationship with peak area is good.The regression equation was Y=23259X+945(R^2=0.9999,n=6),with good reproducibility and average recovery was 100.00%,and the RSD was 1.03%(n=9).The amount of gambogic acid in 6 batches of gamboge from different origins accounts for the total amount of medicinal materials.The average content was 21.39%.Conclusion:The HPLC method in this study is stable,reliable,scientific and simple.It has stronge specificity,and can be used for quantitative determination of gambogic acid in gamboge medicinal materials,and is used for quality evaluation and quality control of ganboge.The system laid the foundation.