温室中患白粉病与健康草莓植株根际原核生物群落的比较研究

Study on Prokaryotic Communities in Rhizospheres of Powdery Mildew-Infected and Non-Infected Strawberry in Greenhouse by High-Through Sequencing Technology

【目的】比较温室大棚中患白粉病的草莓(DRZ)与健康草莓(HRZ)植株根际土壤原核生物(细菌和古菌)群落的多样性与结构差异。【方法】基于土壤原核生物16S rRNA基因V3~V4变异区通用引物序列,应用高通量测序技术进行比较分析。【结果】在97%的相似水平下2个样本共获得OTU个数为2631,涵盖了2域2界39门82纲161目326科600厲的原核生物;与健康样本相比,患病土壤原核生物的OTU数目及多样性指数均较低;在门分类水平上,变形菌门、放线菌门、酸杆菌门和拟杆菌门均为2个样本的优势菌群,两者群落种类大致相同,但群落相对丰度均发生显著变化(P<0.01),同时首次发现了患病植株根际土壤中古菌的门类相对丰度显著高于健康植株;在属分类水平上,分析了2个样本中相对丰度Top 50的菌群,表明原核生物群落结构和相对丰度均发生了显著变化(P<0.01),其中假单胞菌属、链霉菌属和“norank类群”可能是潜在的拮抗菌类群,沃尔巴克氏体属和立克次体属可能是寄生于温室草莓白粉病传播媒介中的类群。【结论】阐明了温室中患白粉病草莓与健康草莓根际原核生物的多样性变化,并解释了病害发生和传播的原因及草莓食用的安全问题,对防控白粉病的发生和快速筛选草莓白粉病拮抗菌提供理论依据。

[Objective]The persent paper aimed to compare prokaryotic communities diversity and structure in the rhizosphere of strawberry infected with powdery mildew and non-infected plants in the greenhouse.[Method]The analysis was based on the variation of 16S rRNA gene V3-V4 region and performed by high-through sequencing platform.[Result]A total number of 2631 operational taxonomic units(OTUs)were obtained from 2 samples under the similarity level of 97%,including 2 domains,2 kingdoms,39 phyla,82 classes,161 orders,326 families,and 600 genera.The results showed that the infection resulted in reduced number of operational taxonomic units(OTUs)and diversity index when compared with the healthy specimen.Proteobacteria,Actinobacteria,Acidobacteria,Bacteroidetes were dominants in the two specimens at the phylum level.The community OTUs of 2 specimens was the same,but the abundance of community composition significantly different(P<0.01).At the same time,we found that the relative abundance of archaea in the rhizosphere of strawberry infected with powdery mildew was significantly higher than that of non-infected plants at the phylum level.At the genus level,we analyzed the relative abundance of Top 50 communities,and the results showed that the prokaryotic community structure and relative abundance had significantly difterence(P<0.01)in 2 specimens.The Pseudomonas,Streptomyces and norank groups might be potential antagonistic microbial populations t and the Wolbachia and Rickettsia might be associated with the spread of strawberry powdery mildew in the Greenhouse.[Conclusion]This study analyzed the prokaryotic communities diversity and structure in the rhizosphere of strawberry infected with powdery mildew and non-infected plants in the greenhouse,the causes of strawberry powdery mildew and the safety of strawberry consumption.Finally,it could provide 8633卷a theoretical basis for effective prevention and control of strawberry powdery mildew and rapid screening of antagonistic bacteria.