胆囊癌患者血清sVEGF-C和CEA水平变化及其与组织病理学特征的关系探讨

Correlation of serumvascular endothelial growth factor-C and carcinoembryonic antigen levels to prognosis in patients with gallbladder cancer

目的研究血清血管内皮生长因子-C(sVEGF-C)和癌胚抗原(CEA)水平与胆囊癌患者临床和病理学特征的关系。方法2011年2月~2018年1月收治的93例胆囊癌患者,采用双抗体夹心酶联免疫吸附法检测血清sVEGF-C水平,采用放射免疫分析法检测血清CEA水平,分析血清sVEGF-C和CEA水平与胆囊癌患者临床和病理学特征的关系。结果不同肿瘤浸润程度(T分期)、肿瘤淋巴转移程度(N分期)、肿瘤远处转移(M分期)的胆囊癌患者血清sVEGF-C和CEA水平相差显著(P<0.05);中位随访22个月,在93例患者中死亡48例(51.6%),生存45例(48.4%);死亡组血清sVEGF-C水平为(84.9±20.7)pg/mL,显著高于生存组[(56.4±18.9)pg/mL,P<0.05],血清CEA水平为(138.7±49.6)μg/L,显著高于生存组[(76.8±27.4)μg/L,P<0.05];多因素Logistic回归分析显示,临床病理分期(OR=6.658,95%CI=1.800~24.632)及血清sVEGF-C(OR=4.005,95%CI=1.292~12.415)和CEA(OR=3.170,95%CI=1.482~6.849)水平是影响胆囊癌患者预后的独立危险因素(P<0.05)。结论胆囊癌患者血清sVEGF-C和CEA水平显著升高,可能与不良预后相关,值得积极的深入研究。

Objective The purpose of this study was to investigate the correlation of serumvascular endothelial growth factor-C(sVEGF-C)and carcinoembryonic antigen(CEA)levels to prognosis in patients with gallbladder cancer.Methods 93 patients with gallbladder cancer were included in this study between February 2011 and January 2018,and serum sVEGF-C levels were detected by double antibody-sandwich enzyme-linked immunosorbent assay,and serum CEA levels were detected by radioimmunoassay.The correlation of serum sVEGF-C and CEA levels to the prognosis of patients with gallbladder cancer was analyzed.Results Serum sVEGF-C and CEA levels were significantly different in gallbladder cancer patients with different tumor infiltration(Tstage),lymphatic metastasis(N stage),distant metastasis(M stage)and clinopathological stage(P<0.05);during 22 month follow-up period,45 survived(48.4%)and 48 died(51.6%);serum sVEGF-C level in the dead group was(84.9±20.7)pg/mL,significantly higher than[(56.4±18.9)pg/mL,P<0.05],and serum CEA level was(138.7±49.6)μg/L,significantly higher than[(76.8±27.4)μg/L,P<0.05]in the survival group;multivariate Logistic regression analysis showed that clinopathological stage(OR=6.658,95%CI=1.800-24.632),serum sVEGF-C(OR=4.005,95%CI=1.292-12.415)and CEA(OR=3.170,95%CI=1.482-6.849)were the independent risk factors affecting the prognosis of patients with gallbladder cancer(P<0.05).Conclusion Serum sVEGF-C and CEA levels increase greatly in patients with gallbladder cancer,which might impact the poor prognosis and warrants further investigation.