复配及多层包埋植物乳杆菌微胶囊的制备及表征

Preparation and Characterization of Lactobacillus plantarum Microcapsules by Compounding and Multi-layer Encapsulation

本研究目的是制备及表征高效保护植物乳杆菌的微胶囊,并对复配及多层包埋效果进行探讨。采用大豆分离蛋白、乳清分离蛋白、海藻酸钠等多种壁材复配包埋植物乳杆菌,并以明胶作为外层壁材,多层包埋制备微胶囊,进行连续240 min的体外消化道实验。结果显示:复配及多层包埋制备的微胶囊包埋率均超过95.14%,抗冻干存活率超过93.25%,粒径均小于2.00 mm,体外消化道试验中多层包埋的植物乳杆菌存活率达到84.41%以上,其中明胶-大豆分离蛋白多层微胶囊的效果最好,其在体外消化道试验中存活率达到了85.73%,且外层光滑,结构紧致。采用复配及多层包埋可有效提高植物乳杆菌的存活率,为益生菌的高效利用奠定了基础。

The purpose of this study was to prepare and characterize microcapsules for the efficient protection of Lactobacillus plantarum,and to discuss the compound encapsulation method and multi-layer encapsulation method. Lactobacillus plantarum was implanted with various wall materials such as soy protein isolate,whey protein isolate and sodium alginate,and microcapsules were prepared by multilayer encapsulation of gelatin as the outer wall material for 240 min in vitro digestive tract experiments. The results showed that the encapsulation rates of microcapsules prepared by compounding and multi-layer embedding were over 95.14%,the survival rate of antifreeze-dried was over 93.25%,and the particle size was less than 2.00 mm. The survival rate of multi-layered Lactobacillus plantarum in the in vitro digestive test was above 84.41%,and the gelatin-soy protein isolate multi-microcapsule had the best effect,the survival rate reached 85.73% in vitro gastrointestinal test,and the outer layer was smooth and the structure was compact. The combination of multi-layer and multi-layer encapsulation could effectively improve the survival rate of Lactobacillus plantarum,which laid a foundation for the efficient use of probiotics.