直穗小檗果粉对STZ诱导的Ⅰ型糖尿病大鼠糖脂代谢的调节作用

Effect of Berberis dasystachya Maxim. Fruit Powder on Glucose and Lipid Metabolisms in STZ-Induced TypeⅠDiabetic Rats

直穗小檗果实在青海地区民间具有较悠久的食用与药用历史,为了探讨直穗小檗果实对STZ诱导糖尿病大鼠的糖脂代谢的调节作用与相关机制,本研究采用链脲霉素(STZ)诱导的Ⅰ型糖尿病大鼠模型,灌胃给予大鼠0.4、1.6 g/kg两个剂量组的直穗小檗果粉28 d后,通过测定大鼠体重,血糖、糖原、血脂水平、脂代谢酶活性及体内抗氧化活性等指标,研究直穗小檗果粉对STZ诱导的Ⅰ型糖尿病大鼠的糖脂代谢的调节活性。实验结果表明:直穗小檗不同剂量(0.4和1.6 g/kg)的果粉给药后,相比模型组大鼠体重分别可增幅16.29%和27.02%;在给药最后一天(第28 d),低剂量和高剂量果粉组血糖相比模型组分别降低25.17%和26.93%;相比模型组,果粉高剂量组可显著增加糖尿病大鼠血清胰岛素水平1.047倍;果粉高剂量组相比模型组大鼠四项指标,总胆固醇(TC)降低了41.17%,甘油三酯(TG)降低了33.52%,低密度脂蛋白(LDL)降低了57.30%和极低密度脂蛋白(VLDL)降低了32.85%;对高密度脂蛋白(HDL)具有调节上升作用(1.44倍)。研究结果还表明,直穗小檗果粉能够使患病大鼠的肝酯酶(HL)和脂蛋白酯酶(LPL)酶活性增加,进而调节其脂代谢指标;同时,当患糖尿病大鼠灌胃给予低剂量和高剂量直穗小檗果粉治疗28 d后,相比模型组大鼠,血清中丙二醛(MDA)水平分别减少56.65%和29.87%;血清中的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、还原型谷胱甘肽(GSH-Px)活性,通过低剂量组直穗小檗果粉治疗后分别增幅88.16%、17.52%和63.43%;通过高剂量组直穗小檗果粉治疗后分别增加1.46倍、1.36倍和1.45倍;病理切片结果表明,直穗小檗果粉能一定程度地修复STZ对胰岛造成的损伤。说明直穗小檗果粉能通过缓解氧化应激来改善胰岛β细胞的损伤,从而达到治疗Ⅰ型糖尿病的作用。

Berberis dasystachya Maxim.represents a prominent and characteristic plant species,indigenous to the Qinghai-Tibet plateau,in order to investigate the regulation effect on glucose and lipid metabolisms and related mechanism of Berberis dasystachya Maxim.powder on STZ-induced diabetic rats,the hypoglycemic and hypolipidemic functions of the Berberis dasystachya Maxim.Fruit powder( BDF) on diabetic rats induced by streptozotocin( STZ) were investigated.Results showed that a decrease in diabetes could be observed after treating rats atdose of 0.4,1.6 g/kg for 28 days.On day 28,compared to MC group,administration of BDF at different dosages( 0.4,1.6 g/kg) resulted in an increase in bodyweight of the diabetic rat by 16.29%and 27.02%,respectively;a significant amelioration in blood glucose levels in diabetic rats could be observed,with blood glucose level reductions by 25.17% and 26.93%,respectively( vs.MC).BDF at a dose of 1.6 g/kg caused reduced levels of TC( 41.17%),TG( 33.52%),LDL( 57.30%),VLDL( 32.85%) and raised levels of HDL( 1.44 times)( vs.MC). Meanwhile,the fruit of Berberis dasystachya M.ameliorated the activities of HL and LPL to lower the lipid levels.It was found that after different dose( 0.4,1.6 g/kg) of BDF treatment,the content of MDA was markedly decreased( 56.65%,29.87%) in serum in STZ-induced diabetic rats( vs.MC).BDF at a dose of 0.4 g/kg could significantly increase the activity of CAT( 88.16%),SOD( 17.52%) and GSH( 63.43%) in serum,while the BDF at a dose of 1.6 g/kg could markelly raise the activities of CAT( 1.46 times),SOD( 1.36 times) and GSH( 1.45 times) in serum in STZ-induced diabetic rats( vs.MC).It is indicated that the BDF functioned as an effective anti-diabetes component and it may protect the β-cells of the pancreatic islet through attenuating oxidative stress.