非小细胞肺癌RAGE/S100P与STAT3/Pim1表达及意义

EXPRESSION OF RAGE/S100P AND STAT3/PIM1 IN NON-SMALL CELL LUNG CANCER AND ITS SIGNIFICANCE

目的探讨非小细胞肺癌(NSCLC)组织晚期糖基化终末产物受体(RAGE)/S100P、信号转导和转录激活因子3(STAT3)表达及其意义。方法收集NSCLC及其癌旁组织标本各40例,应用Western blot方法和RT-PCR方法检测其RAGE、S100P、STAT3和Pim1的表达,并分析不同临床病理特征病人的表达差异。结果肺癌组织中RAGE蛋白表达明显低于癌旁正常组织,两组比较差异有统计学意义(t=5.756,P<0.05);S100P、STAT3和Pim1蛋白在肺癌组织中表达明显高于癌旁正常组织,两组比较差异有统计学意义(t=2.414~10.141,P<0.05)。RT-PCR结果显示,与癌旁正常组织相比,RAGE mRNA在癌组织中表达明显减少(t=21.530,P<0.05),而S100P、STAT3、Pim1 mRNA在癌组织中表达明显增多(t=10.430~13.640,P<0.05)。RAGE mRNA表达量在腺癌组织中高于鳞癌组织,在高中分化组织中表达高于低分化组织,在伴有淋巴结转移的癌组织中表达明显低于不伴有淋巴结转移癌组织,差异有统计学意义(t=2.157~2.908,P<0.05)。S100P mRNA在鳞癌组织中的表达高于腺癌组织,在低分化组织中表达高于高中分化组织,在伴有淋巴结转移癌组织中的表达高于不伴有淋巴结转移癌组织,差异有统计学意义(t=2.132~2.590,P<0.05)。Pearson相关性分析显示,NSCLC组织RAGE表达与S100P表达、STAT3表达呈负相关(r=-0.430、-0.417,P<0.05),STAT3表达与S100P表达、S100P表达与Pim1表达、Pim1表达与miR-21表达呈正相关(r=0.324~0.337,P<0.05)。结论RAGE、S100P及STAT3、Pim1可能参与NSCLC的发生发展。

Objective To investigate the expression of receptor for advanced glycation end products(RAGE)/S100 cal-ciumbinding protein P(S100P)and signal transducer and activator of transcription 3(STAT3)in non-small cell lung cancer(NSCLC)tissue and its significance.Methods NSCLC and paracancerous tissue samples each were collected from 40 patients and were tested for the expression of RAGE,S100P,STAT3,and Pim1 using Western blot and RT-PCR;meanwhile,the samples were analyzed for expression differences between patients with different clinicopathological characteristics.Results The protein expression of RAGE in cancerous tissue was significantly lower than that in normal paracancerous tissue(t=5.756,P<0.05).The protein expression of S100P,STAT3,and Pim1 in cancerous tissue was significantly higher than that in normal paracancerous tissue(t=2.414-10.141,P<0.05).The RT-PCR results showed that the cancerous tissue had significantly reduced mRNA expression of RAGE(t=21.530,P<0.05)but significantly increased mRNA expression of S100P,STAT3,and Pim1(t=10.430-13.640,P<0.05)as compared with normal paracancerous tissue.The mRNA expression levels of RAGE were significantly higher in adenocarcinoma tissue,highly and moderately differentiated tissues,and cancerous tissue without lymph node metastasis than in squamous cell carcinoma tissue,poorly differentiated tissue,and cancerous tissue with lymph node metastasis,respectively(t=2.157-2.908,P<0.05).The mRNA expression levels of S100P were significantly lower in adenocarcinoma tissue,highly and moderately differentiated tissues,and cancerous tissue without lymph node metastasis than in squamous cell carcinoma tissue,poorly differentiated tissue,and cancerous tissue with lymph node metastasis,respectively(t=2.132-2.590,P<0.05).A Pearson’s correlation analysis showed that RAGE expression was negatively correlated with the expression of S100P and STAT3 in NSCLC tissue(r=-0.430,-0.417,respectively,P<0.05),while the expression of STAT3,S100P,and Pim1 was positively correla-ted with the expression of S100P,Pim1,and miR-21,respectively(r=0.324-0.337,P<0.05).Conclusion RAGE,S100P,STAT3,and Pim1 may be involved in the development and progression of NSCLC.