铽掺杂碳点的制备及其抑菌性能

Preparation of terbium doped carbon dots and their antibacterial capacity against Escherichia coli

以马铃薯为原料,通过一步水热法合成碳点,利用稀有金属铽对碳点进行表面修饰,制备铽掺杂碳点(Tb-CDs),通过透射扫描电镜、紫外光谱、红外光谱、荧光光谱等对Tb-CDs进行表征,并研究Tb-CDs在光催化条件下对大肠埃希菌的抑菌作用和对大肠埃希菌做荧光标记的可行性。结果显示,Tb-CDs颗粒小,粒径均匀,分散性好,荧光性强,因其表面富含羧基和羟基等,具有较好的水溶性。在光催化条件下,Tb-CDs对大肠埃希菌具有较好的抑菌作用。当Tb-CDs的质量浓度为0.6 mg·mL^-1,可见光照射60 min时,其对大肠埃希菌的抑菌率可达100%。此外,Tb-CDs能有效地对大肠埃希菌进行荧光标记。

In this study,carbon dots were synthesized by one-step hydrothermal method using potato as raw material,and Tb-doped carbon dots(Tb-CDs)were prepared by surface modification of carbon dots with rare metal terbium.The Tb-CDs were characterized by scanning transmission electron microscope,ultraviolet spectrum,infrared spectrum,and fluorescence spectrum,and the antibacterial effect of the Tb-CDs against Escherichia coli was explored,along with the feasibility of fluorescence label of E.coli by Tb-CDs.It was showed that the prepared Tb-CDs had small uniform particle size,good dispersibility and strong fluorescence.Due to the rich carboxyl groups and hydroxyl groups in the surface,the Tb-CDs had good water solubility.The Tb-CDs had good antibacterial activity against E.coli under photocatalytic conditions.When the concentration of Tb-CDs was 0.6 mg·mL^-1,the antibacteria rate against E.coli could reach 100% under 60 min of irradiation.Besides,the prepared Tb-CDs could effectively label E.coli with fluorescence.