非密闭系统中芦笋老茎的水解特性研究

Study on hydrolysis characteristics of asparagus old stem in non-closed system

非密闭系统可为有机固体废物的生物水解提供兼氧环境,有效提高纤维素水解效率。探讨蔬菜废弃物芦笋老茎在非密闭系统中的水解情况,考查接种量和曝气操作对纤维素水解和腐殖质类物质生成的影响。结果表明:接种纤维素菌剂可加速芦笋老茎的分解,且以接种比0.3%为最优,芦笋老茎最高的总固体分解率和纤维素降解率分别为75.43%和51.03%。红外光谱和三维荧光光谱揭示了水解过程中纤维素和腐殖质类物质的演化特征。腐殖化指数(HIX)和荧光指数(FI)表明,接种菌剂和增加曝气可以进一步提高水解产物的腐殖化程度。水解产物的HIX与发芽指数呈正相关,12 d后水解液中最高的发芽指数为93.2%,说明这种非密闭兼氧水解过程可独立应用于芦笋老茎的减量化处理。

The non-closed system can provide a facultative environment for the microbial hydrolysis of organic solid waste,and can effectively improve the hydrolysis efficiency of cellulose.In the present study,the feasibility of hydrolyzing asparagus old stem in a non-closed system was explored,and the effects of inoculum and aeration on the hydrolysis process and the formation of humic substances were explored.The results showed that the inoculation of cellulose bacteria accelerated the decomposition of old stems of asparagus.When the inoculation ratio was 0.3%,the maximum decomposition rates of total solids and cellulose from asparagus old stem were 75.43%and 51.03%,respectively.Infrared spectroscopy and three-dimensional fluorescence spectroscopy were used to reveal the evolution characteristics of cellulose and humic substances during hydrolysis.The humification index(HIX)and fluorescence index(FI)showed that inoculation and aeration could improve the hydrolysis and the degree of humification.The HIX of the hydrolysate was positively correlated with the germination index(GI),and the highest GI of the hydrolysate was 93.2%after 12 days.It showed that this non-closed facultative hydrolysis process could be applied independently to realize the reduction of vegetable straw.