QuEChERS-HPLC-MS/MS法测定保健药酒中的28种那非类物质的含量

Determination of 28 nafils substances content in health medicinal liquor by QuEChERS-HPLC-MS/MS

建立了保健药酒中28种那非类物质的QuEChERS-HPLC-MS/MS检测方法,样品使用甲醇溶解,经N-丙基乙二胺净化后,经色谱柱分离,在电喷雾离子化正离子模式下,以多反应监测(MRM)模式检测,以保留时间和特征离子对定性,外标法定量。结果表明,28种目标物在10~200μg/L范围内均呈良好的线性关系,相关系数(R2)≥0.9920,加标回收率为90.3%~112.1%,相对标准偏差(RSD)为1.8%~5.3%,检出限为0.01~0.12 mg/L,定量限为0.03~0.36 mg/L。该方法定性定量准确,操作简便,适用于保健药酒中那非类物质的检测与确证。

A QuEChERS-HPLC-MS/MS method for simultaneous determination of 28 nafils substances in health medicinal liquor was developed.The samples were dissolved in methanol,purified by N-propylethylenediamine,separated by chromatographic column,detected using electrospray ionization in the positive ion mode with multi-reaction monitoring(MRM)mode,quantified by retention time and characteristic ion pairs,and quantified by external standards.The results showed that the 28 targets had a good linear relationship in the range of 10-200μg/L,and the correlation coefficient(R2)≥0.9920.The adding standard recovery rate was 90.3%-112.1%,the relative standard deviation(RSD)was 1.8%-5.3%,the limits of detection was 0.01-0.12 mg/L,and the lowest quantitation limit was 0.03-0.36 mg/L.The established method was accurate and simple,which was suitable for the detection and confirmation of nafils substances in health medicinal liquor.