摘要
OBJECTIVE:To observe the effects of New Dayuan powder(NDYP)on Methicillin-resistant Staphylococcus aureus(MRSA)biofilms and the embedded bacteria in vitro.METHODS:2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide(XTT)assays were used to study the effects of NDYP on developing MRSA biofilms:100μL of bacterial culture and100μL drug solution were added to wells of96-well plates.After 24 h of incubation,the plates were washed and XTT-phenazine methyl sulfate(PMS)was added to enable counting of the number of live bacteria in biofilms using a microplate reader.XTT assays were also used to explore the effects of NDYP on mature MRSA biofilms:100μL of bacterial culture were added to wells of 96-well plates.Bacteria were cultured in the plates for 24 h,and then drug solution was added.The plates were cultured for another 24 h,and then XTT-PMS was added to detect the number of live bacteria in the biofilms.Scanning electron microscopy(SEM)was used to observe the effects of NDYP on mature MRSA biofilms:washed and sterilized glass coverslips were added to 24-well plates.Bacterial culture was added.After 24 h of incubation,drug solution was added.After another 24 h of incubation,the samples were observed by SEM.RESULTS:XTT assays showed that the number of live bacteria in both developing and mature MRSA biofilms decreased significantly(P<0.01)after the administration of NDYP.SEM images showed that NDYP could destroy the structure of the bacteria and resulted in uneven thickness of MRSA biofilms.CONCLUSION:In vitro,NDYP has obvious inhibitory effects on the formation of MRSA biofilms and on mature biofilms.
OBJECTIVE: To observe the effects of New Dayuan powder(NDYP) on Methicillin-resistant Staphylococcus aureus(MRSA) biofilms and the embedded bacteria in vitro.METHODS: 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide(XTT) assays were used to study the effects of NDYP on developing MRSA biofilms: 100 μL of bacterial culture and100 μL drug solution were added to wells of96-well plates. After 24 h of incubation, the plates were washed and XTT-phenazine methyl sulfate(PMS) was added to enable counting of the number of live bacteria in biofilms using a microplate reader. XTT assays were also used to explore the effects of NDYP on mature MRSA biofilms: 100 μL of bacterial culture were added to wells of 96-well plates. Bacteria were cultured in the plates for 24 h,and then drug solution was added. The plates were cultured for another 24 h, and then XTT-PMS was added to detect the number of live bacteria in the biofilms. Scanning electron microscopy(SEM) was used to observe the effects of NDYP on mature MRSA biofilms: washed and sterilized glass coverslips were added to 24-well plates. Bacterial culture was added. After 24 h of incubation, drug solution was added. After another 24 h of incubation, the samples were observed by SEM.RESULTS: XTT assays showed that the number of live bacteria in both developing and mature MRSA biofilms decreased significantly(P < 0.01) after the administration of NDYP. SEM images showed that NDYP could destroy the structure of the bacteria and resulted in uneven thickness of MRSA biofilms.CONCLUSION: In vitro, NDYP has obvious inhibitory effects on the formation of MRSA biofilms and on mature biofilms.
基金
Support by the Fundamental Scientific Research Funds for China Academy of Chinese Medical Sciences(Independent Topic Selection Project)
