Bcl-2、MIF蛋白在老年牙龈癌组织中的表达与临床意义

Role of Bcl-2 and MIF proteins in senile patients with gingival cancer

目的:探讨老年牙龈癌组织中Bcl-2蛋白、巨噬细胞移动抑制因子(MIF)蛋白的表达及其临床意义。方法:选取山东省青岛疗养院2015年1月-2017年9月确诊的62例老年牙龈癌组织标本作为实验组,正常牙龈组织31例作为对照组,采用免疫组织化学染色检测2组标本中Bcl-2蛋白、MIF蛋白的表达,分析不同TNM临床分期、是否发生颈淋巴结转移、不同组织学分化程度的老年牙龈癌组织中的Bcl-2蛋白、MIF蛋白差异。采用SPSS 16.0软件包对数据进行χ^2检验。结果:实验组牙龈组织标本中Bcl-2蛋白、MIF蛋白的阳性表达率分别为67.74%和70.97%,对照组分别为16.13%、22.58%,2组比较差异有统计学意义(P<0.05);在不同分化程度的牙龈癌组织间,Bcl-2蛋白的阳性表达率具有统计学差异(P<0.05);在不同分化程度的牙龈癌组织、是否发生颈淋巴结转移的牙龈癌组织间,MIF蛋白的阳性表达率具有统计学意义差异(P<0.05)。结论:Bcl-2、MIF蛋白在老年牙龈癌组织中表达上调,与肿瘤分化程度、颈淋巴结转移具有一定关系。

PURPOSE:To investigate the expression of Bcl-2 protein and macrophage migration inhibitory factor(MIF)protein in gingival carcinoma of the elderly and its clinical significance.METHODS:Sixty-two gingival cancer tissue specimens in the elderly diagnosed in our hospital from January 2015 to September 2017 were selected as the experimental group,and 31 normal gingival tissues were selected as the control group.Immunohistochemical staining was used to detect the expression of Bcl-2 protein and MIF protein in two groups.The differences of Bcl-2 protein and MIF protein in different clinical stages,cervical lymph node metastasis and histological differentiation were analyzed.The data were analyzed with SPSS 16.0 software package for Chi-square test.RESULTS:The positive expression rates of Bcl-2 protein and MIF protein in the gingival cancer samples of the experimental group were 67.74%and 70.97%,respectively.The positive expression rates of Bcl-2 protein and MIF protein in the gingival tissues of the control group were 16.13%and 22.58%,respectively.The difference was statistically significant(P<0.05).The positive expression rate of Bcl-2 protein in gingival carcinoma tissues with different degrees of differentiation was statistically significant(P<0.05).In different degrees of differentiation,gingival carcinoma with cervical lymph node metastasis,there was significant difference in the positive expression rate of MIF protein in gingival cancer tissues(P<0.05).CONCLUSIONS:The expression of Bcl-2 and MIF protein is up-regulated in gingival cancer tissues of the elderly,which has certain relationship with the degree of tumor differentiation and cervical lymph node metastasis.