不同剂量姜黄素对干热环境热射病大鼠肺损伤的保护作用

The protective effects of different dosages of curcumin on lung injury of rats in dry heat environment

目的探讨不同剂量姜黄素对干热环境热射病大鼠肺损伤的保护作用。方法将50只SD大鼠随机(随机数字法)分为5组(n=10):常温对照组(NC),干热对照组(DHC),3个不同剂量姜黄素预处理的干热组(50mg/kg、100mg/kg、200 mg/kg)。NC、DHC组给予生理盐水灌胃,姜黄素预处理组给予不同剂量的姜黄素每天1次灌胃,连续7d。第8天除NC组外,其余4组大鼠转移至西北特殊环境人工实验舱内进行实验,环境温度(41±0.5℃),湿度(10±1)%。实验的第150分钟达到热射病状态,麻醉后取材。肺组织进行HE染色并进行肺损伤病理学评分,观察肺湿干质量比(W/D),测定支气管肺泡灌洗液(BALF)中总蛋白、白细胞数及中性粒细胞数等肺损伤指标。结果肺损伤病理学评分、W/D、BALF中总蛋白、白细胞数及中性粒细胞数在NC组最低,DHC组最高,差异有统计学意义(P<0.01)。随着姜黄素剂量的增加,肺损伤病理学评分、W/D、BALF中总蛋白、白细胞数及中性粒细胞数均呈下降趋势。姜黄素不同剂量组之间及其与NC组、DHC组均差异有统计学意义(P<0.01)。相关性分析提示,肺病理损伤评分与肺组织W/D比值、肺泡BALF中总蛋白含量、白细胞数、中性粒细胞数存在相关性(相关系数分别为r=O.879,r=0.935,r=0.916,r=O.88O,P<0.01)。结论不同剂量的姜黄素可对干热环境热射病大鼠的肺损伤发挥保护作用,姜黄素在干热环境热射病肺损伤的防治方面可能具有重要的临床应用价值。

Objective To study the protective effects of different concentrations of curcumin on lung injury of rats in dry heat environment.Methods Fifty Sprague-Dawley rats were randomly(random number)divided into five groups(n=10 each group):normal control group(NC),dry heat control group(DHC),and three different concentrations of curcumin pretreated dry-heat groups(50 mg/kg,100 mg/kg,and 200 mg/kg).Rats in the NC and DHC groups were given a gavage of normal saline,and rats in the curcumin pretreatment groups were given a gavage of curcumin with different concentrations,once a day for 7 consecutive days.At 8th day,all groups except for the NC group were transferred to the climate cabin(The Simulated Climate Cabin for Special Environment of Northwest of China),with a temperature of(41±0.5)and relative humidity of(10±1)%.Rats in each group were anesthetized and sampled after reaching the state of heat stroke at 150 min,and same done to the NC group.Lung tissues were harvested and pathological changes were observed by HE staining.Lung wet/dry(W/D)weight ratio was detected and lung injury indicators such as total protein,white blood cell count and neutrophil count in bronchoalveolar lavage fluid(BALF)were also determined.Results The lung injury pathology score,W/D weight ratio,total protein,white blood cell count and neutrophil count in BALF were the lowest in the NC group,and the highest in the DHC group,with significant difference(P<0.01).With the increase of the concentration in the curcumin pretreatment groups,lung injury pathology score,W/D weight ratio,total protein,white blood cell count and neutrophil count in BALF were all declined.There were significant differences among the different concentration groups of curcumin and the NC and DHC groups(P<0.01).Correlation analysis indicated that lung injury scores were correlated with W/D weight ratio of lung tissue,alveolar total protein content in BALF,white blood cell count,and neutrophil count(correlation coefficient r-0.879,r=0.935,r=0.916,and r=0.880,respectively,P<0.01).Conclusions Pretreatment with different concentrations of curcumin can exert protective effects on lung injury of heat stroke rats in dry heat environment.Curcumin may have important clinical value in prevention and treatment of lung injury caused by heat stroke in dry heat environment.