血吸虫毛蚴动态自动识别系统在尼龙绢集卵孵化法中的应用价值

Application value of a dynamic automatic identification system in Schistosoma japonicum miracidium hatching test

目的探讨血吸虫毛蚴动态自动识别系统在常规尼龙绢集卵孵化法观察毛蚴中的价值。方法在牛粪中加入不同数量的日本血吸虫新鲜虫卵,分为低感染组、中感染组和高感染组,以不含虫卵的牛粪为阴性对照组,比较不同组毛蚴识别系统与肉眼观察法检测毛蚴的效率、准确率;同时观察不同毛蚴数量和不同检测时间毛蚴识别系统检出毛蚴数量。结果该系统可自动识别毛蚴,阳性检测率和检测效率均明显高于肉眼观察法;尤其在低感染组,毛蚴识别系统阳性检测显著高于肉眼观察法(χ^2=10.769,P=0.002)。毛蚴识别系统检测1 min可用于现场检测粪便样本。结论血吸虫毛蚴动态自动识别系统可有效提高尼龙绢集卵孵化法毛蚴检测效率和准确率,可替代肉眼观察法用于血吸虫病现场查病及相关研究。

Objective To evaluate the value of a dynamic automatic identification system in routine miracidium hatching test with nylon gauzes.Methods Different quantities of fresh Schistosoma japonicum eggs were added to bovine fecal samples and divided into the low-infection group,medium-infection group and high-infection group,while the bovine feces without S.japonicum eggs served as negative controls.The detection efficiency and accuracy were compared between the identification system and manual detection in different groups.Results The identification system can automatically identify S.japonicum miracidium.The detection rate and efficiency of S.japonicum miracidium in bovine fecal samples were both higher by using the identification system than by manual detection.Notably in the low-infection group,the identification system had a significantly higher rate of detection of S.japonicum miracidium than manual detection(χ^2=10.769,P=0.002).The identification system completed the detection of bovine fecal samples in the field within 1 min.Conclusions The dynamic automatic identification system may effectively improve the detection efficiency and accuracy of routine miracidium hatching test with nylon gauzes,and it may replace manual detection to be used in the field schisotsomiasis examinations and related researches.