B7S1在胃癌中的表达及其临床意义

Expression and clinical significance of B7S1 in gastric cancer

目的分析B7超家族成员1(B7 superfamily member 1,B7S1)在胃癌和癌旁组织中的表达差异,探讨B7S1与胃癌患者临床病理特征和预后的相关性。方法收集78例手术切除胃癌患者标本,采用逆转录-聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)和免疫组织化学法检测B7S1在胃癌及其癌旁组织中的表达,并分析B7S1表达与胃癌患者临床病理特征和预后相关性。结果胃癌组织中B7S1 mRNA的相对表达量高于癌旁组织(P<0.05);胃癌组织中B7S1蛋白表达阳性率(74.36%)高于癌旁组织(11.54%),差异有统计学意义(P=0.001);胃癌组织中B7S1蛋白表达与肿瘤直径、病理分期、T分期和淋巴结转移相关(P值分别为0.006、0.002、0.011和0.001),而与性别、年龄、肿瘤部位和M分期无关(P>0.05)。单因素和Cox多因素生存分析均提示B7S1阳性表达为胃癌患者预后不良的危险因素。结论胃癌组织中B7S1 mRNA相对表达水平和蛋白表达阳性率高于癌旁组织,胃癌组织中B7S1蛋白阳性表达意味着患者不良病理特征和不良预后,提示B7S1可能参与胃癌的恶性进展。

Objective To analyze the difference of expression of B7 superfamily member 1(B7S1)in gastric cancer and adjacent cancer tissues,and to explore the relationship between B7S1 expression and the clinicopathological characteristics and the prognosis of gastric cancer patients.Methods Reverse transcription-polymerase chain reaction(RT-PCR)and immunohistochemistry were performed to investigate the expression of B7S1 in 78 cases of gastric cancer tissues and adjacent tissues.The correlation of B7S1 expressions with the clinicopathological characteristics and prognosis of the patients was analyzed.Results The results showed that cancer tissues relative expression of B7S1 mRNA was higher than that of adjacent tissues,and the difference was statistically significant(P<0.05).The results showed that positive rate of B7S1 protein expression in gastric cancer tissues was significantly higher than that of adjacent tissues,74.36%and 11.54%,the difference was statistically significant(P=0.001).Chi-square analysis showed that the expression of B7S1 protein was associated with tumor diameter(P=0.006),pathological-stage(P=0.002),T-stage(P=0.011),and lymph node metastasis(P=0.001).There were no relationship with gender,age,tumor site,and M-stage(P>0.05).B7S1 protein expression was correlated with the overall survival rate for gastric cancer patients.Both univariate and Cox multivariate survival analysis suggested that B7S1 positive expression was a risk factor for poor prognosis in patients with gastric cancer.Conclusions The relative expression level of B7S1 mRNA and the positive rate of protein expression in gastric cancer tissues are higher than those in adjacent tissues.The positive expression of B7S1 is correlated with the poor clinicopathological characteristics and prognosis.We speculate that B7S1 may be involved in the malignant progression of gastric cancer.