木鳖子制霜炮制前后HPLC指纹图谱比较研究

Comparison of the HPLC Fingerprints of Momordicae Semen Before and After Frost-like Processing

目的:建立木鳖子及木鳖子霜的HPLC指纹图谱,并进行比较研究。方法:采用HPLC法建立10批不同产地木鳖子炮制前后的指纹图谱,采用HPLC法以Agilent Zorbax SB-C18柱(250 mm×4.6 mm,5μm)为色谱柱,乙腈-0.2%磷酸为流动相,梯度洗脱,流速:1.0 mg·ml^-1,柱温:25℃,波长:203 nm。采用中药相似度评价软件进行相似度分析,通过聚类分析法对炮制前后木鳖子指纹图谱进行分析。结果:建立了木鳖子炮制前后HPLC指纹图谱共有模式,以齐墩果酸峰为参照峰,以木鳖子炮制前后HPLC图谱中13个共有峰作为特征峰,经相似度分析,与对应对照指纹图谱相似度均大于0.9,且炮制前后指纹图谱相似度差异较小。通过聚类分析可基本区分木鳖子生品及炮制品。结论:该方法可用于木鳖子药材及木鳖子霜的指纹图谱分析,为完善木鳖子及木鳖子霜质量标准提供科学依据,且能有效区分木鳖子药材及木鳖子霜。

Objective:To establish an HPLC fingerprint of Momordicae Semen before and after frost-like processing.Methods:An HPLC method was adopted to establish the fingerprint of saponins in 10 batches of Momordicae Semen from different origins before and after frost-like processing.The chromatographic column was Agilent Zorbax SB-C18 column(250 mm×4.6 mm,5μm).Methanol-0.2%glacial acetic acid was used as the mobile phase with gradient elution at the flow rate of 1.0 mg·ml^-1,the column temperature was 25℃,and the wavelength was 203 nm.Similarity analysis was carried out by using similarity evaluation software of traditional Chinese medicine,and cluster analysis method was used to analyze the fingerprint of Momordicae Semen before and after the processing.Results:The common pattern of HPLC fingerprint of Momordicae Semen before and after the processing was established.Oleanolic acid peak was taken as the reference peak,and there were 13 common peaks as the characteristic peaks in HPLC.Through the similarity analysis,the similarity of fingerprints of the corresponding controls was greater than 0.9,and the fingerprints were similar before and after the processing.The raw and processed products of Momordicae Semen can be distinguished by the cluster analysis.Conclusion:The method can be used for the fingerprint analysis of Momordicae Semen before and after processing,which provides scientific basis for improving the quality standards,and can effectively distinguish the raw and processed products of Momordicae Semen.