miR-26b、miR-30e在老年性痴呆症患者血清中的表达及临床意义

Expressions and clinical significance of miR-26b and miR-30e in serum of patients with Alzheimer disease

目的研究miR-26b、miR-30e在老年性痴呆患者血清中的表达特点和临床意义。方法选取2015年6月至2017年9月江门市新会区第三人民医院收治的200例老年痴呆症患者进行研究,其中阿尔茨海默病患者100例为AD组,轻度认知功能障碍(MCI)患者100例为MCI组,另选取100例同期在本院的健康体检者为NC组,通过实时荧光定量PCR(qRT-PCR)检测各组血清中miR-26b、miR30e的表达水平,比较三组之间一般临床资料以及甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、同型半胱氨酸(Hcy)、脂联素(ANP)含量;Pearson相关分析AD患者血清miR-26b、miR-30e与各生化指标的相关性;二分类Logistic回归分析影响AD发生的危险因素。结果相对于NC组,AD组、MCI组血清中Hcy、LDL-C含量显著升高,ANP含量显著降低(P<0.05);AD组、MCI组血清中miR-26b表达水平分别为0.51(0.15,0.86)、0.79(0.21,1.14)显著低于NC组[1.06(0.42,1.32)](P<0.05),AD组血清中miR-26b表达水平明显低于MCI组(P<0.05);AD组、MCI组血清中miR30e表达水平分别为1.27(0.53,1.72)、1.02(0.35,1.65),明显高于NC组[0.86(0.43,1.32)](P<0.05),AD组血清中miR-30e表达水平明显高于MCI组(P<0.05)。miR-26b相对表达水平与ANP值呈正相关(r=0.626,P<0.05),与LDL-C、Hcy值呈负相关(r=-0.517、-0.523,P<0.05);miR-30e表达水平与LDL-C、Hcy值呈正相关(r=0.536、0.644,P<0.05),与ANP值、miR-26b表达水平呈负相关(r=-0.732、-0.563,P<0.05)。Logistic回归分析发现,Hcy、LDL-C、miR-26b、miR-30e是AD发生的独立危险因素(P<0.05),ANP是AD的保护因素(P<0.05)。结论AD患者血清中miR-26b表达显著下调,miR-30e表达水平显著上调,miR-26b、miR-30e二者均与AD的发生发展有关。

Objective To study the expression characteristics and clinical significance of miR-26b and miR-30e in serum of patients with Alzheimer disease(AD).Methods A total of 200 patients with senile dementia admitted to our hospital from June 2015 to September 2017 were selected for the study.Among them,100 patients with AD were assigned as AD group,the other 100 patients with mild cognitive impairment(MCI)were assigned as MCI group.Another 100 patients who took health examination in our hospital during the same period were assigned as NC group.Real-time fluorescence quantitative polymerase chain reaction(qRT-PCR)was used to detect the expression levels of miR-26b and miR-30e in serum of each group.The general clinical data and the contents of triglyceride(TG),total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),high density lipoprotein cholesterol(HDL-C),homocysteine(Hcy)and adiponectin(ANP)were compared among the three groups.Pearson correlation analysis was used to analyze the correlations between serum miR-26b,miR-30e and biochemical parameters in AD patients.The risk factors of AD were analyzed by binary logistic regression.Results Compared with NC group,the levels of serum Hcy and LDL-C in AD group and MCI group increased significantly,while the level of ANP decreased significantly(P<0.05).The levels of serum miR-26b in AD group and MCI group were 0.51(0.15,0.86)and 0.79(0.21,1.14)respectively,which were significantly lower than that in NC group 1.06(0.42,1.32)(P<0.05).The levels of serum miR-26b in AD group were significantly lower than that of MCI group(P<0.05).The level of serum miR-30e in AD and MCI groups were 1.27(0.53,1.72)and 1.02(0.35,1.65),which were significantly lower than those in NC group 0.86(0.43,1.32)(P<0.05).The serum level of miR-30e in AD group was significantly higher than that in MCI group(P<0.05).The expression level of miR-26b was positively correlated with ANP(r=0.626,P<0.05),and negatively correlated with LDL-C and Hcy(r=-0.517,-0.523,P<0.05).The expression level of miR-30e was positively correlated with LDL-C and Hcy values(r=0.536,0.644,P<0.05),while negatively correlated with ANP values and the expression level of miR-26b(r=-0.732,-0.563,P<0.05).Logistic regression analysis showed that Hcy,LDL-C,miR26b and miR-30e were independent risk factors for AD(P<0.05).And ANP was protective factor for AD(P<0.05).Conclusions The expression of miR-26b in serum of AD patients is significantly down-regulated,the expression of miR-30e is significantly up-regulated.Both miR-26b and miR-30e are related to the occurrence and development of AD.