CTGF对牙槽骨缺损修复区正畸牙移动影响的实验研究

Experimental study of CTGF on orthodontic tooth movement in alveolar bone defect repair area

目的:探讨CTGF(结缔组织生长因子)对正畸牙在牙槽骨缺损修复区内移动过程中压力侧牙周组织改建的影响。方法:选择28只新西兰大白兔,通过完整拔除下颌左侧第一磨牙,同时植入Bio-Oss骨粉加Bio-Gide膜,建立骨缺损修复模型。8周后,随机选取4只兔进行植骨区取材,制作组织学切片,评价成骨效果。将剩余24只兔随机分为两组:CTGF实验组和生理盐水对照组,每组12只。以下颌中切牙为支抗牵引左侧下颌第二磨牙向近中移动,将CTGF及生理盐水每隔1 d分别注射到实验组及对照组的骨缺损修复区域的舌侧骨粘膜下,分别在加力后的第7、14、21、28 d每组各随机处死3只动物,测量牙移动距离。制作组织学切片,釆用TRAP染色法计数压力侧破骨细胞的数目。结果:正畸牙在实验组牙槽骨修复区内的移动量在第7、14.21.28 d均大于对照组(P均<0.05),差异具有统计学意义。两组牙压力侧破骨细胞数目在第14 d达到高峰,在第14、21和28 d实验组破骨细胞数目高于对照组(P均<0.05),差异具有统计学意义。结论:正畸牙在牙槽骨缺损修复区内早期移动时,结缔组织生长因子能够增加压力侧破骨细胞的数量,有利于正畸牙齿的移动。

Objective:To investigate the effect of CTGF(connective tissue growth factor)on the periodontal tissue remodeling of orthodontic tooth during the movement of the alveolar bone defect repair zone.Methods:Twenty-eight New Zealand white rabbits were selected and the bone defect repair model was established by completely removing the first molar on the left side of the mandible and implanting Bio-Oss bone powder plus Bio-Gide membrane.After 8 weeks・4 rabbits were randomly selected for bone grafting・and histological sections were made to evaluate the osteogenic effect.The remaining 24 rabbits were randomly divided into two groups:CTGF experimental group and saline control group,with 12 rats in each group.The following mid-maxillary incisors are the traction-resisting traction of the left mandibular second molar to the near-middle.CTGF and normal saline were injected into the lingual bone mucosa of the bone defect repair area of the experimental group and the control group every 1 day.Three animals were randomly sacrificed in each of the groups on the 7th・14th,21st,and 28th after the afterburning,and the tooth movement distance was measured.Histological sections were made and the number of osteoclasts on the pressure side was counted by TRAP staining.Results:The amount of orthodontic tooth movement in the alveolar bone repair area of the experimental group was higher than that of the control group at the 7th,14th,21st and 28th day(P<0.05),and the difference was statistically significant.The number of osteoclasts on the pressure side of the two groups reached a peak on the 14th day.On the 14th・21st and 28th day,the number of osteoclasts in the experimental group was higher than that in the control group(P<0.05),and the difference was statistically significant.Conclusion:When the orthodontic tooth moves early in the alveolar bone defect repair area・connective tissue growth factor can increase the number of osteoclasts on the pressure side,which is beneficial to the movement of orthodontic teeth.