对具有一个共同亲本的杂种F1育性差异的初步研究

Preliminary Study on Fertility Difference of F1 Hybrids Based on A Common Parent

【目的】对具有一个共同亲本的杂种F1所表现出的育性差异进行了研究。【方法】观察了F1的育性、减数分裂配对情况;采用SLAF-Seq-BSA(specific-locus amplified fragment sequencing and bulked segregant analysis)技术,对与育性有关系的基因进行关联分析;对不育F1及其两个亲本的花药的转录组进行了分析。【结果】在不同播期下,正反交杂种F1表现出稳定的不育特性,其自交结实率低于0.5%(国内法)。雄性不育的014-459/川麦55和可育的014-459/绵08-9杂种F1减数分裂时染色体行为相似。SLAF分析将不育相关基因在染色体上的关联区域限定在2D染色体上的3个区域,转录组分析将亲本和F1之间表达有差异的基因确定出来,结合SLAF分析和转录组分析,找到了F1不育的育性相关基因。【结论】减数分裂染色体行为异常不是014-459和普通小麦的杂种F1表现不育的原因。F1不育是一种新的不育类型,初步确定与F1表现出不育的关联基因是2D01G027900,GO注释号是0009753,其主要功能是对茉莉酸起响应。

【Objective】Study on fertility difference of F1 hybrids based on a common parent.【Method】Fertility of F1 and chromosome pairing at meiosis metaphase Ⅰ were observed;SLAF-Seq-BSA(specificlocus amplified fragment sequencing and bulked sergeant analysis)was used to mapping the chromosome regions causing male sterility.【Result】Reciprocal crossing hybrid F1s between 014-459 and cultivar chuanmai 55 were stably male sterility under different sowing time and the selfing rate arrived at less than 0.5%(internal selfing rate)in individual exceptional sowing time.Similar chromosome behaviour during meiosis was also observed in both male sterility(014-459/Chuanmai 55)and male fertility F1(014-459/Mian 08-9)hybrids.The results of SLAF-Seq-BSA showed that three intervals of chromosome 2D were associated with male sterility.Differential expression genes between parents and male sterility F1 were determined by transcriptome analysis and genes associated with F1 male sterility were found out combined with SLAF-Seq and transcriptome analysis.【Conclusion】Defectious meiosis chromosome behaviour was not the reason causing male sterility in hybrids F1,it is a new type of male sterility.One gene likely associated with target character was 2D01G027900 with GO number 0009753 which was response to jasmonic acid.