椰子ZF-HD基因家族的鉴定及生物信息学分析

Identification and Bioinformatics Analysis of Coconut ZF-HD Gene Family

ZF-HD蛋白是一类只存在于植物体内且含有锌指结构域的转录因子家族,其不仅能够调节植物的生长发育,且在植物响应逆境过程中也起着重要的作用。本研究通过比对已开发的椰子基因组数据,共鉴定出20个椰子ZF-HD蛋白。采用生物信息学的方法,对其基因结构、蛋白理化性质、蛋白质保守结构域、超二级结构、系统进化树及表达谱进行分析。结果显示:椰子ZF-HD蛋白多为碱性蛋白,且主要定位于细胞核、叶绿体及线粒体中。椰子ZF-HD基因家族可分为6个亚族,每个亚家族都具有相似的结构域和超二级结构。motif搜索分析显示,CoMIF亚族只含有锌指结构域保守序列,其他亚族均含有锌指结构域序列与同源异形盒结构域序列。表达谱分析显示,CoZHD18、CoZHD20在测序的各组织中表达较少,其他家族成员在各组织中的表达量具有差异性。

ZF-HD protein is a family of transcription factors that contain zinc finger domains and only exist in plants.It not only regulates the growth and development of plants,but also plays an important role in floristic response to stress.In this study,20 ZF-HD proteins of coconut were identified by comparison with the developed coconut genome database.Bioinformatics methods were used to analyze its genic structure,protein physicochemical properties,protein conserved domains,motifs,phylogenetic trees and expression profiles.The analysis showed that the coconut ZF-HD proteins are mostly basic protein.And they are mainly located in the nucleus,chloroplast and mitochondria.The coconut ZF-HD gene family could be divided into 6 subfamilies,each of which had similar structural domains and super secondary structures.The motif search analysis showed that the CoMIF subfamily only contained the zinc finger domain conserved sequence,and the other subfamilies contained the zinc finger domain sequence and the homeobox domain sequence.And expression profiling revealed that CoZHD18 and CoZHD20 were not detected in the sequenced tissues,and the expression levels of other family members in each tissue were different.