水稻OsPR10A的表达特征及其在干旱胁迫应答过程中的功能

Expression Characterization of Rice OsPR10A and Its Function in Response to Drought Stress

利用免疫印迹(WB)分析了水稻(Oryzasativa)OsPR10A在其不同生长时期、不同组织部位及多种非生物逆境胁迫下的表达特征,发现OsPR10A在干旱、盐胁迫以及茉莉酸甲酯(MeJA)和脱落酸(ABA)诱导下表达量明显升高,表明该蛋白可能在干旱和盐胁迫应答过程中发挥作用。为证明这一推测,我们构建了OsPR10A超表达载体,经农杆菌介导转化水稻,获得超表达OsPR10A的纯合株系。田间表型观察表明,转基因株系株高变矮、穗长变短、结实率降低。用20%PEG6000在水稻种子萌发过程中进行干旱处理,结果显示,OsPR10A超表达株系的根长和芽长均显著高于野生型,证明超表达OsPR10A可增强水稻萌发期耐旱性。该研究有助于增进人们对水稻OsPR10A功能的了解。

In this study, we surveyed the expression patterns of rice OsPR10A protein by using western blot analysis for protein samples extracted from different tissues at different developmental stages and a variety of abiotic stresses. The expression of OsPR10A was upregulated significantly under drought, salt stress and hormone treatments such as methyl jasmonate and abscisic acid, which suggests that OsPR10A protein plays a role in drought and salt stress response. To test this hypothesis, we constructed a vector for overexpression of OsPR10A protein and obtained homozygous transgenic lines via Agrobacterium-mediated rice transformation. Phenotypic investigation in field experiments revealed shorter plant height and spike length as well as lower seed setting rate in transgenic lines than controls. At seed germination stage, drought stress treatment was applied by using 20% PEG6000. Root and shoot length was significantly longer in OsPR10A overexpression lines than the wild-type control, which supports that overexpression of OsPR10A protein enhanced the drought tolerance of rice during germination. The study increases our understanding of the function of rice OsPR10A protein.