黄芪多糖对X线辐射骨髓间充质干细胞成脂分化的影响

Effects of astragalus polysaccharide on radiation-induced adipogenic differentiation of bonemarrow mesenchymal stem cells

目的研究中药黄芪有效成分黄芪多糖(APS)对X射线照射人骨髓间充质干细胞成脂分化的影响。方法用50μg/mL的APS干预2 Gy X射线照射的人骨髓间充质干细胞,专用培养基诱导后,通过油红O法染色并计数观察干细胞的脂滴数量及大小,Western blot检测成脂相关蛋白(C/EBPα和PPAR-γ)表达变化。结果X线辐射后骨髓间充质干细胞分化为脂肪细胞数量减少,成脂细胞中脂滴的面积明显减少(P<0.05),提前给予黄芪多糖的骨髓间充质干细胞组脂滴面积较单纯辐射组增加(P<0.05)。同样,X线辐射后,成脂相关蛋白过氧化物酶增殖物激活受体-γ(PPAR-γ)和重组人CCAAT增强子结合蛋白(CEBPα)表达降低,而给予黄芪多糖干预后,PPAR-γ和CEBPα表达均升高(P<0.05)。结论X线可损伤人骨髓间充质干细胞的定向成脂分化功能,黄芪多糖对X线辐射人骨髓间充质干细胞成脂分化功能具有一定的保护作用。

Objective To study the effects of astragalus polysaccharide(APS)on adipogenic differentiation of human bone marrow mesenchymal stem cells(BMSCs)irradiated by X-ray.Methods Human bone marrow mesenchymal stem cells irradiated by 2 Gy X-ray were intervened with 50μg/mL of APS.The number and size of lipid droplets of stem cells were observed by oil red O staining and the expressions of CEBPαand PPAR-γwere detected by Western blot after induction with special medium.Results The number of adipocytes differentiated from bone marrow mesenchymal stem cells decreased,and the area of lipid droplets in adipocytes decreased significantly after irradiation(P<0.05).The area of lipid droplets in the bone marrow mesenchymal stem cells treated with APS in advance increased compared with that in radiation alone(P<0.05).Similarly,the expressions of PPAR-γand recombinant human CCAAT enhancer binding protein(CEBPα)decreased after X-ray irradiation,while the expressions of PPAR-γand CEBPαincreased after the intervention of APS(P<0.05).Conclusion X-ray can damage the directional adipogenic differentiation of human bone marrow mesenchymal stem cells,and APS has a protective effect on the adipogenic differentiation of human bone marrow mesenchymal stem cells after X-ray irradiation.