化学模式识别分析白及不同采收期次生代谢产物动态变化特征

Characteristics research on dynamic changes of secondary metabolites in Bletilla striata for different harvest time combined with chemical pattern recognition

为研究白及栽培品不同采收期特征差异次生代谢产物及其动态变化规律。采用高效液相色谱法(HPLC)建立白及指纹图谱并测定gastrodin、militarine含量,利用主成分分析(PCA)及最小偏二乘分析(PLS-DA)对指纹图谱进行化学模式识别,从16个共有峰中识别7个特征化学成分为不同采收季节主要特征差异成分。利用高效液相-高分辨质谱技术(HPLC-HRMS)鉴定为葡萄糖苷类成分(gastrodin)、苄酯类成分(blestroside、militarine、gymnosideⅤ)以及菲类成分(4-甲氧基菲-2,7-二醇、benzylhydrophenanthrene 3、coelonin)。结果显示7个主要差异成分随季节呈现不同动态变化规律,gastrodin和militarine在不同采收期含量差异较大。本研究为指导白及采收时期及内在质量评价提供理论依据。

To analyze the characteristic secondary metabolites and its dynamic change rule of cultivated Bletilla striata collected from different harvest time.The chemical fingerprints of 24 Bletilla striata samples collected from different harvest time was established by high performance liquid chromatography(HPLC)and the content of gastrodin、militarine were determined.Principal components analysis(PCA)and partial least squares-discriminate analysis(PLS-DA)were carried out on the HPLC fingerprints,16 common peaks were marked in the HPLC fingerprint,furthermore,7 characteristic secondary metabolites were found and identified(glucosides:gastrodin;benzyl ester:blestroside,militarine,gymnosideⅤ;phenanthrenes:4-methoxyphenanthrene-2,7-diol,coelonin,benzylhydrophenanthrene 3)by HPLC-high resolution mass spectrometry(HPLC-HRMS).Results indicated that these 7 chemical compounds were the characteristic secondary metabolites and showed different dynamic changes with seasons.The results were demonstrated to be very useful for guiding harvest time and quality assessment of Bletilla striata.