摘要
本试验以29株核桃内生真菌为研究对象,采用总还原力(FRAP法)及DPPH·清除试验评价其代谢产物的抗氧化能力,从中筛选出高活性菌株,并对该菌株发酵液进行浓缩萃取,研究各萃取物的抗氧化活性。结果表明,供试29株菌的代谢产物都表现出一定的抗氧化活性,其中菌株LTS-6-6(Pyrenochaeta)为高活性菌株。该菌株发酵液乙酸乙酯萃取物(EAE)显示了较强的抗氧化活性,对DPPH·的EC 50为7μg/mL,总还原力为527.59±10.66 mg V C/g,总酚和总黄酮含量分别达到641.14±7.50 mg没食子酸/g、100.30±8.44 mg芦丁/g。该结果表明,核桃内生真菌代谢产物具有一定的抗氧化活性,是寻找天然抗氧化活性物质的良好资源。
In order to study the antioxidant capacity of endophytic fungi,the total antioxidant capacity(FRAP method)and DPPH·assay were used to evaluate the antioxidant capacity of the 29 endophytic fungi metabolites and screen a high strain.The active strain LTS-6-6 was extracted to study the antioxidant capacity of the extractant of each phase.The results showed that the metabolites of 29 strains showed a certain antioxidant capacity,and 9 strains with strong DPPH·clearance rate(clearance rate≥50%)accounted for 31.03%of the tested strains.Among them,the strain LTS-6-6(Phrenochaeta)was a highly active strain.The ethyl acetate extract(EAE)of the fermentation broth showed strong antioxidant activity with the EC 50 of 7μg/mL and a total antioxidant capacity of 527.59±10.66 mg V C/g.The total phenolic and total flavonoid contents were respectively reached 641.14±7.50 mg gallic acid/g,100.30±8.44 mg rutin/g.The results indicated that the endophytic fungal metabolites of walnut had certain antioxidant capacity and were good resources for finding natural antioxidant active substances.
作者
庞俊倩
赵鑫丹
郝苑汝
高飞
翟梅枝
PANG Jun-qian;ZHAO Xin-dan;HAO Yuan-ru;GAO Fei;ZHAI Mei-zhi(Walnut Research Centre,College of Forestry,Northwest A&F University,Yangling 712100,China)
出处
《天然产物研究与开发》
CAS
CSCD
北大核心
2020年第1期40-46,共7页
Natural Product Research and Development
基金
国家自然科学基金(31370552)
关键词
核桃内生真菌
总还原能力
DPPH·
抗氧化活性
endophytic fungi of walnut
total reducing ability
DPPH·
antioxidant activity