血浆反应素环状卡片试验与梅毒螺旋体明胶颗粒凝集试验在梅毒抗体临床检测中应用效果的比较研究

A comparative study on the effect of plasma reactive hormone ring card test and treponema pallidum gelatin particle agglutination test in the clinical detection of syphilis antibody

目的比较血浆反应素环状卡片试验(RPR)与梅毒螺旋体明胶颗粒凝集试验(TPPA)在梅毒抗体临床检测中的应用效果。方法选取四川锦欣妇女儿童医院2017-2018年治疗的梅毒患者80例,所有患者均行RPR以及TPPA检测。比较RPR以及TPPA的检测结果。结果80例患者检测中出现2例前带情况,分别为一期梅毒和二期梅毒,将两例患者血清标本进行稀释,稀释比例为1:2,1:4,1:8,1:16,1:32,1:64,稀释完成后再次进行RPR检测,该一期梅毒患者血清在1:16稀释情况下呈阳性,该二期梅毒患者血清在1:32稀释情况下呈阳性。结论RPR在梅毒临床检测中的敏感性不高,不适于疾病的诊断,可作为治疗检测及初次筛查使用,而TPPA检测准确率更高,临床可根据患者具体情况应用。

Objective To compare the effect of RPR and TPPA in the clinical detection of syphilis antibody.Methods A total of 80 syphilis patients were treated in Sichuan Jin Xin Women’s and Children’s Hospital from 2017 to 2018 were selected.RPR and TPPA tests were performed on all patients.The results of RPR and TPPA were compared.Results In 80 patients,there were two cases of pre band,one was primary syphilis and the other was secondary syphilis.The serum samples of the two patients were diluted in the proportion of 1:2,1:4,1:8,1:16,1:32,1:64.After dilution,RPR was detected again.The serum of the primary syphilis patients was positive in the dilution of 1:16,and the serum of the secondary syphilis patients was positive in the dilution of 1:32.Conclusion RPR is not sensitive in clinical detection of syphilis and is not suitable for disease diagnosis.It can be used as treatment detection and initial screening,while TPPA detection has a higher accuracy and can be used according to the specific situation of patients.