体外同期放疗化疗抵抗结直肠癌细胞模型的建立及其mRNA表达谱的变化探讨

Establishment of colorectal cancer cell model resistant to concurrent chemoradiotherapy in vitro and the changes of its mRNA expression profile

目的构建体外同期放疗、化疗抵抗结直肠癌细胞模型,探讨其mRNA表达谱的变化。方法模拟临床高剂量疗法获到HCT116同期放疗、化疗后残癌细胞株(HCT116 colorectal cancer radiation resistance cell,HCT116 CRR),应用mRNA芯片比较模型细胞与其野生型细胞的mRNA表达谱的变化情况,初步筛选与结直肠癌同期放疗、化疗抵抗相关的mRNA。结果成功构建HCT116 CRR细胞株,与野生型HCT116细胞相比,mRNA芯片共检测出变化差异在2倍以上的mRNA共3832条(13.88%)。其中,2倍以上上调的共1847条;2倍以上下调的共1985条;10倍以上上调的共35条;10倍以上下调的共50条。结论与其野生型HCT116细胞相比,HCT116 CRR细胞株的mRNA表达谱发生显著变化,差异性表达的mRNA可能参与了结直肠癌放疗、化疗抵抗产生的分子调节过程。

Objective To establish the colorectal cancer cell model resistant to concurrent chemoradiotherapy in vitro and explore the changes of messenger ribonucleic acid(mRNA)expression profile.Methods HCT116 colorectal cancer radiation resistance cell(HCT116 CRR)was obtained by simulating clinical high-dose therapy.mRNA microarray was used to compare the mRNA expression profiles of model cells and wild-type cells,and to preliminarily screen the mRNA related to concurrent chemoradiotherapy resistance in colorectal cancer.Results The HCT116 CRR cell line was successfully constructed.Compared with the wild-type HCT116 cells,there were 3832(13.88%)mRNAs with a difference of more than 2 times,of which 1847 mRNAs were more than 2 times up-regulated,1985 mRNAs were more than 2 times down-regulated,35 mRNAs were more than 10 times up-regulated and 50 mRNAs were more than 10 times down-regulated using mRNA microarray.Conclusion Compared with its wild-type HCT116 cells,the HCT116 CRR cell line has a significant change of mRNA expression profile.The differentially expressed mRNA may be involved in the molecular regulation of radiotherapy and chemotherapy resistance in colorectal cancer.