屏南县羊口疮病的诊断和PCR流行病学调查

Diagnosis and PCR Epidemiological Investigation of Sore Mouth Disease in Pingnan County

羊口疮病是屏南县羊养殖过程中常见的疾病之一,制约了屏南县养羊产业的健康发展。为了确定屏南县临床羊口疮病的病原以及了解该病在屏南县的流行情况,为该病的科学防治提供依据。采用PCR方法检测了12份县域内临床羊口疮病的病原,并随机采集了县域内6家规模养羊场(户)217份羊只血样进行羊口疮病的流行病学调查。结果表明:12份病例的病变组织、血样提取的病毒基因组DNA样品均扩增出约500 bp片段,与预期片段大小一致,明确了引起屏南县临床羊口疮病的病原为羊口疮病毒(ORFV);屏南县6家规模养羊场(户)的羊口疮病毒(ORFV)感染率为14.63%~33.33%,平均感染率为25.99%;抽样调查的群体中有羊口疮临床病症的羊只均为1~3月龄羔羊,各养殖场临床发病率为0~16.33%,平均发病率为9.69%,低于羊群羊口疮病毒(ORFV)感染率。

The sore mouth disease is one of the common diseases in sheep breeding in Pingnan County,which has restricted the healthy development of sheep breeding industry in Pingnan County.By determining the pathogen of clinical sore mouth disease in Pingnan County and understanding the prevalence of the disease in Pingnan County,it would provide a basis for the scientific prevention and treatment of the disease.PCR was used to detect the pathogens of 12 cases of clinical sore mouth disease in the county,and a total of 217 sheep blood samples were randomly collected from 6 large-scale sheep farms(households)in the county for epidemiological investigation.The results showed that the DNA samples of viral genome extracted from the pathological tissue and blood samples of the 12 cases were amplified to approximately 500 bp fragments,which were consistent with the expected fragment size,so it was identified that the pathogen causing clinical sore mouth disease in Pingnan County was ORFV.The infection rate of ORFV in 6 large-scale sheep farms(households)in Pingnan County was 14.63%-33.33%,with an average infection rate of 25.99%.Among the sampled groups,all the sheep with clinical symptoms of sore mouth disease were 1-3 month-old lambs,and the clinical incidence rate of each farm was 0-16.33%,with an average incidence rate of 9.69%,which was lower than the infection rate of ORFV.