白芨多糖的超声辅助提取工艺及抗氧化活性研究

Ultrasound-assisted Extraction Process and Antioxidant Activity of Bletilla Striata Polysaccharide

白芨多糖在医药领域及食品科学方面有诸多应用,具有良好的发展前景。本实验采用超声辅助提取法从干燥白芨块茎中提取白芨多糖,利用单因素实验和正交实验法寻求较适提取条件并进行多糖结构鉴定,对比分析传统水提法和超声提取法制得的白芨多糖的体外抗氧化能力。根据数据可得较适提取工艺条件:超声功率为400 W,料液比为1:35 g/m L,超声时间为50 min,此条件下白芨多糖得率达到8.18%±0.04%。超声辅助法制得的白芨多糖对DPPH·、·OH的清除率明显高于传统水提法制得的白芨多糖。两种提取方法制得的多糖对ABTS+·清除率无明显差异,均无O2-·清除能力。

Bletilla striata polysaccharide has many applications and development prospects in medicine and food science. In this experiment, the polysaccharides from Bletilla striata were extracted by ultrasonic-assisted extraction method. The single factor experiment and orthogonal experiment were used to find the best conditions and then identified its structure. The traditional water extraction method and ultrasonic extraction method were used to compare antioxidant activity of Bletilla striata polysaccharide. According to the data, the optimal extraction process conditions were obtained: the ultrasonic power was 400 W, the ratio of material to liquid was 1:35 g/m L, and the ultrasonic time was 50 min. The yield of the polysaccharide was 8.18% + 0.04% under this condition. The scavenging rate of DPPH· and ·OH of Bletilla striata polysaccharide by ultrasonic-assisted extraction method was higher than by traditional water extraction method. The polysaccharides obtained by the two extraction methods showed no significant difference in ABTS+· clearance rate, and none of them had O2-· clearing ability.