钆塞酸二钠增强MRI对奥沙利钳诱导C57BL/6小鼠肝功能损伤的评估价值

Value of the gadoxetic acid-enhanced magnetic resonance imaging on oxaliplatin-induced liver function injury in C57BL/6 mice

目的探讨钆塞酸二钠增强MRI对奥沙利钳诱导C57BV6小鼠肝功能损伤的评估价值。方法雄性无特定病原体C57BI>6小鼠40只,6周龄,体质量19-23 g0使用随机数字随机分成:对照组、实验A组、实验B组和实验C组,各10只。对照组腹腔注射生理盐水,实验组每周2次腹腔注射奥沙利钳,实验A组、B组、C组分别连续注射2、4、6周。测量增强MRI肝月旦特异期T1弛豫时间、首过快速上升期中的增强斜率百分比(ESP)O检测血清胆红素和白蛋白,计算白蛋白胆红素(ALBI)评分。病理组织染色观察肝组织损伤和纤维化。受试者工作特征(ROC)曲线评价ALBI评分、肝胆特异期T1弛豫时间、ESP诊断肝功能损伤的价值。结果实验组(A组、B组和C组)中16只小鼠纳入变性组(不伴有纤维化的肝细胞变性),14只纳入肝纤维化组。肝纤维化组肝胆特异期T1弛豫时间高于对照组和变性组,差异有统计学意义(均P<0.05)。对照组、变性组、肝纤维化组ESP呈升高趋势,差异有统计学意义(均P<0.05)。与对照组比较,变性组、肝纤维化组ALBI评分均降低,差异有统计学意义(均P<0.05)。肝纤维化组小鼠,ALBI评分、肝胆特异期T\弛豫时间和ESP诊断肝功能损伤的ROC曲线下面积分别为0.734.0.962和0.989。结论钆塞酸二钠增强MRI肝胆特异期T1弛豫时间和ESP可有效评估奥沙利钳诱导的C57BL/6小鼠肝功能损伤。

Objective To investigate the value of gadoxetic acid-enhanced magnetic resonance imaging(MRI)on oxaliplatin-induced liver function injury in C57BL/6 mice.Methods Forty male and six weeks old C57BL/6 mice without specific pathogens were included and the body weght ranged from 19 to 23 g.They were randomly assigned into control group,experimental group A,experimental group B and experimental group C(10 mice/group).The mice in the control group was intraperitoneally injected with saline solution.The mice in the experimental groups were intraperitoneally injected with oxaliplatin twice a week.The experimental group A,B and C were administered for 2 weeks,4 weeks and 6 weeks,respectively.The T1relaxation time on the hepatobiliary phase and the first rapid enhancement slope percentage(ESP)in liver parenchyma were measured and calculated.Serum albumin and bilirubin values were measured and albumin and bilirubin(ALBI)scores were calculated.Pathological staining was used to observe liver tissue damage and fibrosis.The receiver operating characteristic(ROC)curve evaluated the ALBI score,ESP and T]relaxation time on hepatobiliary phase for the diagnosis of liver function.Results Sixteen mice in the experimental groups(including group A,B and C)were included in the hepatic degeneration group(hepatocyte degeneration without fibrosis).Fourteen mice were included in the hepatic fibrosis group.relaxation time on hepatobiliaiy phase in the hepatic fibrosis group was higher than that in the control group and in the hepatic degeneration group.The differences were statistically significant(P<0.05).The ESP of the control group,the hepatic degeneration group and the hepatic fibrosis group was increased,with statistically significant differences(all P<0.05).Compared with the control group,ALBI scores of the hepatic degeneration group and the hepatic fibrosis group were both decreased,with statistically significant differences(P<0.05).In the hepatic fibrosis group,the areas under the curve of ALBI scores,the T1relaxation time on hepatobiliary phase and the ESP were 0.734,0.962 and 0.989,respectively.Conclusion The T1relaxation time on hepatobiliary phase and the ESP of gadoxetic acid-enhanced MRI can effectively evaluate the hepatic function reduction induced by oxaliplatin-induced hepatic tissue injury in C57BL/6 mice.