水稻(Oryza sativa)OsSPR1突变体及过表达株系对锰胁迫的响应

Response of Oryza sativa Os SPR1 Mutant and Its Over-expressed Line to Manganese Stress

为了系统分析水稻OsSPR1基因突变体Osspr1及过表达株系对锰胁迫的响应情况。本研究以水稻短侧根突变体Osspr1及过表达株系为试验材料,研究不同锰胁迫条件下水稻生长,锰吸收和累积量及其抗氧化酶活性的差异。研究结果表明,水稻OsSPR1的过表达株系(OV1)的根系发育和植物生长已完全恢复至野生型水平。在锰胁迫条件下,spr1突变体的地上部和根系的锰含量、锰累积量均显著低于野生型。在高锰(4 mmol/L Mn2+)处理下过表达株系OV1的锰含量和锰累积量显著低于野生型,OV1锰转移系数显著低于野生型。锰胁迫条件下,突变体spr1和过表达材料OV1叶片的抗氧化酶过氧化物酶(peroxidase, POD)、过氧化氢酶(catalase, CAT)、超氧化物歧化酶(superoxide dismutase, SOD)的活性均高于或者显著高于野生型。综合以上研究表明Os SPR1基因突变和过表达均可以提高水稻对锰胁迫的耐受能力。本研究为水稻耐锰新品种的选育提供材料和基因资源。

In this study, a short postembryonic roots mutant(Osspr1), and OsSPR1 over-expressed line were used as experimental materials, research on the differences of rices growth, manganese absorption, accumulation and antioxidant enzyme activities under different manganese stress conditions, and then systematically analyzed that the response of Osspr1 mutant and its over-expressed line to manganese stress. The results showed that the root development and plant growth of over-expressed of OsSPR1 line(OV1) had completely recovered to the wild-type l evel. Under manganese stress, the manganese content and accumulation of spr1 mutant shoot and root were significantly decreased than the wild type. and the manganese content and accumulation of over-expressed line OV1 shoot was significantly lower than that of the wild type under high manganese(4 mmol/L Mn2+) treatment, meanwhile manganese transfer coefficient of OV1 also decreased than the wild type under high Mn(4 mmol/L Mn2+)treatment. The activities of antioxidant enzymes peroxidase(POD), catalase(CAT), superoxide dismutase(SOD) in the leaves of spr1 and OV1 were higher or significantly higher than those of the wild type. The above research showed that the OsSPR1 gene mutation and over-expression could improve the tolerance of rice to manganese stress. This study could provided materials and genetic resources for the breeding of new manganese-tolerant rice varieties.