新型超细晶铜材料植入大鼠子宫中Cu2+释放及对子宫组织的影响

Release of Cu2+ in the uterus of rats by new ultrafine grained copper material and its effect on uterus tissue

目的:研究新型超细晶铜材料与普通铜材料植入大鼠子宫腔的Cu2+释放以及对子宫形态的影响。方法:将普通铜材料(对照组)和新型超细晶铜材料(实验组)铜丝,分别植入12周龄雌性Wistar大鼠左侧子宫,右侧未植入子宫为自身对照。分别在12 h、1 d、3 d、7 d、15 d和30 d处死大鼠,收集外周血清、宫腔灌洗液、子宫和肝脏组织,用电感耦合等离子体原子发射光谱仪(ICP)测定样本中Cu2+的含量,并对子宫组织行常规组织形态学分析。结果:宫腔植入铜材料后,与对照组相比,实验组大鼠血清及肝脏组织中的Cu2+浓度无差异。两组宫腔灌洗液中Cu2+浓度在植入3d时最高,7d、15d时降低且实验组低于对照组(P<0.05);两组子宫组织Cu2+浓度在植入12 h时最高,植入1d、3d时降低(P<0.05),随后趋于平稳,且植入7d、15d时实验组Cu2+浓度低于对照组(P<0.05)。两组子宫组织在植入早期表现为急性无菌性炎症,但实验组组织损伤修复显示更快。结论:新型超细晶铜材料作为宫内节育器新材料,能够在时间和程度上显著降低Cu2+暴释现象,有望成为替代现有铜材料的重要选择。

Objective:To study the Cu2+release of new ultrafine grained copper materials and ordinary copper materials in the uterine cavity,and to analyze its effect on the morphology of uterus of rats.Methods:Ordinary copper material(control group)and new ultra-fine crystal copper material(experimental group)were prepared as wires,and were implanted into the left uterus of 12-weeks-old female Wistar rats.And the right uterus of these rats hadn’t been implanted any material as self-control.The rats were sacrificed at 12 h,or on the 1st d,3rd d,7th d,15th d,and 30th d after the copper material implantated in the rat uterine cavity,and their peripheral serum,uterine lavage fluid,uterus and liver tissues were collected as samples.The concentration of Cu2+in these samples was determined by inductively coupled plasma atomic emission spectrometry(ICP),and the histomorphology of the uterus tissue was analyzed.Results:There were no significant different in the concentration of Cu2+in the serum and liver tissues of rats after implantation of copper material between the experimental group and the control group.The concentration of Cu2+in the uterine lavage fluid of rats on the 3rd day in the two groups after implantation of copper material was the highest,and had decreased on 7th day and 15th day(P<0.05).The concentration of Cu2+in the uterine lavage fluid of rats on the 5th or 7th day after implantation of copper material in the experimental group was significant lower than that of rats in the control group(P<0.05).The concentration of Cu2+in the uterus of rats in the two groups at 12 h after implantation of copper material was the highest,and had decreased on the 1st day and the 3rd day(P<0.05),and then the concentration of Cu2+in the uterus stabilized.The concentration of Cu2+in the uterus of rats on the 7th or the 15th day of rats in the experimental group after implantation of copper material was significant lower than that of rats in the control group(P<0.05).The uterus tissue of rats in both groups showed acute aseptic inflammation early after implantation of copper material,but the tissue damage of rats in the experimental group showed a faster repaired.Conclusion:As a new material for intrauterine device,the new ultra-fine crystal copper material can significantly alleviate the phenomenon of Cu2+burst release,which may become an important alternative to existing copper materials.