单纯疱疹病毒Ⅱ型gE糖蛋白B细胞表位的预测及初步鉴定

Prediction and preliminary identification of B cell epitope for gE glycoprotein of herpes simplex virus typeⅡ

目的探讨单纯疱疹病毒II型gE糖蛋白B细胞表位作为靶点研制ELISA试剂盒候选抗原的可能性.方法采用Emini、IEDB等网络在线分析工具对该基因序列的编码区进行表面可及性、柔韧性、抗原表位、亲水性以及线性B细胞表位的生物信息进行综合分析.筛选出不同预测方法中一致的3条多肽,分别以标准阳、阴性血清进行Dot-blot验证,并以临床血清进行ELISA检测效果的评价.结果单纯疱疹病毒II型gE糖蛋白序列具有18个抗原表位和23个线性B细胞表位.Dot-blot检验肽段结果显示,gE糖蛋白B细胞表位具有较强的抗原反应.临床血清检验结果显示,B细胞表位2#和3#多肽均表现出良好的灵敏度和特异性.结论单纯疱疹病毒II型gE糖蛋白B细胞表位多肽可以作为开发抗体诊断用抗原的备选.

Objective To investigate the potential of gE glycoprotein B cell epitope of HSV II as a target for the development of ELISA kit.Methods Emini and IEDB online analysis tools were used to analyze the surface accessibility,flexibility,linear B cell antigen epitope,hydrophilicity of the protein.Three polypeptides were screened out for dot-blot verification with standard positive serum and standard negative serum respectively,and clinical serum was used to evaluate the ELISA results.Results HSV type II gE glycoprotein sequence has 18 epitopes and 23 linear B-cell epitopes.Dot-blot analysis showed that the B cell epitopes of gE glycoprotein had strong antigen reaction.Clinical serum test showed that 2#and 3#B cell epitopes showed good sensitivity and specificity.Conclusions HSV II gE glycoprotein B cell epitope peptides have the potential to be antigens for the development of antibody diagnosis.